Characteristics of Faecal Microbiota in Korean Patients with Clostridioides difficile-associated Diarrhea
- Author:
Yong Duk JEON
1
;
Hea Won ANN
;
Woon Ji LEE
;
Jun Hyoung KIM
;
Hye SEONG
;
Jung Ho KIM
;
Jin Young AHN
;
Su Jin JEONG
;
Nam Su KU
;
Joon Sup YEOM
;
Dongeun YONG
;
Kyungwon LEE
;
Jun Yong CHOI
Author Information
- Publication Type:Original Article
- From:Infection and Chemotherapy 2019;51(4):365-375
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND:The intestinal microbiota plays an important role in the pathogenesis of Clostridioides difficile-associated diarrhea, and regional and racial characteristics influence the microbiome composition and diversity. We investigated the intestinal microbiome characteristics of patients with C. difficile colitis (CD+) compared to those of patients with colitis not due to C. difficile (CD−), patients with vancomycin-resistant enterococci (VRE) colonization, and healthy controls, in Korea.
MATERIALS AND METHODS:We collected stool samples from 24, 18, 11 and 13 subjects within CD+, CD−, VRE and healthy control groups, respectively. The microbial communities were evaluated by 454-pyrosequencing of bacterial 16s rRNA.
RESULTS:The species richness and microbial diversity were significantly lower in the CD+ group compared to those in healthy controls, but not compared to those in CD− and VRE groups. Phylum-level analysis showed that the proportion of Actinobacteria in the CD+ group was significantly lower than in the healthy control, but was unchanged compared to that in CD− and VRE groups. At the genus level, compared to the healthy group, the CD+ group showed significantly lower proportions of Blautia, Bifidobacterium, Faecalibacterium et al. Compared to the VRE group, the CD+ group showed a significantly higher proportion of Anaerostipes.
CONCLUSIONS:We could identify the intestinal microbiome characteristics of Koreans with C. difficile colitis. It might help to develop microbiome based diagnostic and treatment modalities.
