Quantitative Measurement of D-dimer by VIDAS System in Patients with Suspected DIC.
- Author:
Hyun Kyung KIM
1
;
Kyung Soon SONG
;
Quehn PARK
Author Information
1. Department of Clinical Pathology, College of Medicine, Yonsei University, Seoul, Korea
- Publication Type:Original Article
- Keywords:
VIDAS D-dimer;
latex D-dimer;
Disseminated intravascular coagulation;
Concordance rate
- MeSH:
Agglutination;
Dacarbazine*;
Diagnosis;
Disseminated Intravascular Coagulation;
Fibrin;
Fibrinogen;
Humans;
Latex;
Partial Thromboplastin Time;
Platelet Count;
Prothrombin Time
- From:Korean Journal of Clinical Pathology
1999;19(5):491-495
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: D-dimer, cross-linked fibrin degradation fragment, is an important parameter for the diagnosis of disseminated intravascular coagulation (DIC). The commonly used method for detecting D-dimer levels is latex agglutination, which is rapid but lacks sufficient sensitivity. Recently, quantitative and rapid D-dimer assay have been available for routine laboratory use. METHODS: We compared quantitative D-dimer by VIDAS system with semiquantitative latex D-dimer. Sixty-six patients at risk for DIC were classified as DIC and subclinical DIC based on the modified DIC criteria by Mant et al. VIDAS D-dimer (BioMerieux, Marcyl'Etiole, France), latex D-dimer (Diagnostica Stago, Asnieres, France), fibrinogen (Diagnostica Stago), prothrombin time (Diagnostica Stago), activated partial thromboplastin time (Diagnostica Stago), and platelet count (Sysmex SE9000, Japan) were measured. RESULTS: In 9 patients with documented DIC it was found that VIDAS D-dimer level was markedly elevated (>2,000 ng/mL) in 100%, with the mean D-dimer level being 10,720+/-7,727 ng/mL and that latex D-dimer was markedly elevated in 33.3%. There was no significant difference of VIDAS D-dimer level according to the clinical diagnosis. The concordance rate between VIDAS and latex D-dimer is 33.3% (22/66). Thirty-four (51.5%) of all patients had VIDAS D-dimer level of more than 2,000 ng/mL and latex D-dimer of 1000-2000 ng/mL. There was negative correlation between VIDAS D-dimer levels and APTT (or fibrinogen). CONCLUSIONS: We concluded the quantitative VIDAS D-dimer to be more sensitive than latex D-dimer and to be a useful parameter in diagnosing DIC. It is needed to set cutoff value of D-dimer in order to get more specific information for the diagnosis of DIC.
