HSP60 is required for stemness and proper differentiation of mouse embryonic stem cells
- Author:
Nan Hee SEO
1
;
Eun Hye LEE
;
Jin Hee SEO
;
Hwa Ryung SONG
;
Myung Kwan HAN
Author Information
- Publication Type:Original Article
- From:Experimental & Molecular Medicine 2018;50(3):e459-
- CountryRepublic of Korea
- Language:English
- Abstract: Embryonic stem cells (ESCs) are metabolically distinct from their differentiated counterparts. ESC mitochondria are less complex and fewer in number than their differentiated progeny. However, few studies have examined the proteins responsible for differences in mitochondrial structure and function between ESCs and somatic cells. Therefore, in this study, we aimed to investigate the differences between mitochondrial proteins in these two cell types. We demonstrate that HSP60 is more abundant in mouse ESC mitochondria than in mouse embryonic fibroblasts. Depletion of HSP60 inhibited mouse ESC proliferation and self-renewal, characterized by decreased OCT4 expression. HSP60 depletion also enhanced apoptosis during mouse ESC differentiation into embryoid bodies. Our results suggest that HSP60 expression has an essential role in ESC self-renewal and survival of differentiated cells from ESCs.
