Outer membrane proteins of Salmonella typhimurium as an adjuvant in rabies vaccine
10.7774/cevr.2021.10.2.132
- Author:
Iman Ibrahim NEGM
1
;
Yasser M. RAGAB
;
Aly Fahmy MOHAMED
Author Information
1. The Egyptian Holding Company for the Production of Vaccines, Sera and Drugs, Cairo, Egypt
- Publication Type:Original Article
- From:Clinical and Experimental Vaccine Research
2021;10(2):132-140
- CountryRepublic of Korea
- Language:English
-
Abstract:
Purpose:The objective of the present study was to evaluate the immune-enhancing potential of Salmonella typhimurium outer membrane protein (OMP) and alum as adjuvants towards inactivated Vero cells rabies vaccine (FRV/K2).
Materials and Methods:Six groups of female Sprague Dawley albino rats (10/group) were used in the evaluation of immunogenicity and safety of vaccines and adjuvants. Total immunoglobulin G secreted interferon-gamma (IFN-γ), and the percentage of proliferated CD4+ and CD8+ T cells were measured. Biochemical analysis and histopathological examination were used to test safety profiles.
Results:OMP adjuvanted rabies vaccine (FRV/K2+OMP) (OMP combined locally prepared vaccine) induced significantly higher neutralizing antibodies on day 21 post-vaccination relative to free (FRV/K2) vaccine and alum adsorbed vaccine (FRV/K2+alum) (alum adsorbed locally prepared vaccine). (FRV/K2+OMP) induced a significantly higher level of IFN-γ on day 14 post-vaccination. CD8+ T cells were significantly higher post-vaccination with reference (RV), free (FRV/K2), and (FRV/K2+OMP) than (FRV/K2+alum). On the contrary, CD4+ T cells were significantly elevated post-vaccination with (FRV/K2+alum) at p<0.05. Biochemical analysis and histopathological examination revealed that OMP could be used safely as an adjuvant for the development of more effective rabies vaccines.
Conclusion:Outer membrane proteins adjuvanted rabies vaccines would be beneficial to induce rapid neutralizing antibodies and essential cytokines.
