Optimization of extraction technology of Guizhi shaoyao zhimu decoction
- VernacularTitle:桂枝芍药知母汤提取工艺的优化
- Author:
Shujing ZHENG
1
;
Jia WANG
1
;
De HU
1
;
Yang LI
1
;
Xiangyang LENG
1
;
Shumin WANG
1
Author Information
1. School of Pharmacy，Changchun University of Chine se Medicine，Changchun 130117，China
- Publication Type:Journal Article
- Keywords:
Guizhi shaoyao zhimu decoction;
extraction technology;
analytic hierarchy technology;
entropy weight method
- From:
China Pharmacy
2022;33(2):179-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To optimize the e xtraction technology of Guizhi shaoyao zhimu decoction （GSZD）. METHODS The contents of 9 components in GSZD were determined by HPLC ，such as ephedrine hydrochloride ，pseudoephedrine hydrochloride ， mangiferin，paeoniflorin，liquiritin，5-O-methylvisammioside，glycyrrhizic acid ，cinnamic acid ，6-gingerol. On the basis of single factor experiment ，taking material-liquid ratio ，extraction times and extraction time as inspection factors ，taking the contents of above 9 components and the yield of dry extract as evaluation indicators ，the analytic hierarchy process and entropy weight method were used to determine the composite weight of each index and calculate the comprehensive score ；the extraction technology parameters of GSZD were optimized by Box-Behnken response surface method ，and the validation tests were conducted. RESULTS The composite weight of the contents of ephedrine hydrochloride ，pseudoephedrine hydrochloride ，mangiferin，paeoniflorin， glycyrrhizin，5-O-methylvisa- midol ，glycyrrhizinate，cinnamic acid ，6-gingerol and the yield of dry extract were respectively 0.12，0.10，0.05，0.12，0.14，0.06，0.13，0.15，0.10，0.03. The optimal extraction technology of GSZD is that the ratio of material to liquid is 1 ∶ 14（g/mL），extraction is 2 times，and the extraction time is 3.0 h；average comprehensive score of the 3 verification tests was 95.879，and RSD was 0.50％（n＝3），the deviation from the predicted comprehensive score （94.328）was 1.64％. CONCLUSIONS In this study ，the optimal extraction technology of GSZD is determined.
