Establishment of the fingerprints of Yinhuang solution for inhalation and content determination of phenolic acids
- VernacularTitle:银黄吸入溶液的指纹图谱建立及酚酸类成分的含量测定
- Author:
Jin GAO
1
,
2
;
Cui LI
1
,
2
;
Ruizhuo YIN
2
;
Xincheng MA
2
;
Huiyang WANG
2
;
Chunhui GONG
2
;
Chengyu CHEN
1
,
2
;
Hui CAO
1
Author Information
1. College of Pharmacy，Jinan University，Guangzhou 510632，China
2. Increasepharm （Hengqin） Institute Co.，Limited/National Engineering Research Center for Modernization of Traditional Chinese Medicine New Drug Delivery System Branch/Guangdong Province Engineering Research Center for Aerosol Inhalation Preparation，Guangdong Zhuhai 519000，China
- Publication Type:Journal Article
- Keywords:
Yinhuang solution for inhalation;
high performance liquid chromatography;
fingerprint;
phenolic acids
- From:
China Pharmacy
2022;33(2):160-164
- CountryChina
- Language:Chinese
-
Abstract:
OBJE CTIVE To establish the finger prints for Yinhuang solution for inhalation and determine the contents of neochlorogenic acid ，chlorogenic acid and cryptochlorogenic acid simultaneously. METHODS Using baicalin as reference ，the fingerprints of Yinhuang solution for inhalation were established by high performance liquid chromatography （HPLC）. Relative correction factors of neochlorogenic acid and cryptochlorogenic acid were calculated by slope correction method ，using chlorogenic acid as reference ；the contents of them were calculated according to relative correction factor. The results of quantitative analysis of multi-components by single marker （QAMS）were compared with those of external standard method （ESM）. RESULTS There were 18 common peaks in the fingerprints of 10 batches of Yinhuang solution for inhalation ，and their similarities with reference fingerprint were higher than 0.90. A total of 7 common peaks were identified as baicalin ，neochlorogenic acid ，chlorogenic acid ， cryptochlorogenic acid ，isochlorogenic acid B ，3，5-di-O-caffeoylquinic acid and 4，5-di-O-caffeoylquinic acid. The linear range of neochlorogenic acid ，chlorogenic acid and cryptochlorogenic acid were 0.025 0-1.247 4 μg（r＝0.999 7），0.039 3-1.178 7 μg（r＝ 0.999 9），0.031 6-1.184 1 μg（r＝0.999 9），respectively. RSDs of precision ，reproducibility and stability tests （48 h）were all lower than 1.0％. The average recoveries were 93.92％（RSD＝1.32％ ，n＝6），94.46％（RSD＝1.45％，n＝6），93.93％（RSD＝ 1.57％，n＝6）. Relative correction factors of neochlorogenic acid and cryptochlorogenic acid were 1.068 and 1.233. The contents of neochlorogenic acid and cryptochlorogenic acid determined by QAMS method were 0.301 8-0.386 3 and 0.262 5-0.362 5 mg/mL， respectively. The contents of neochlorogenic acid ，chlorogenic acid and cryptochlorogenic acid by ESM were 0.302 6-0.387 2， 0.231 0- 0.334 0，0.261 6-0.361 3 mg/mL，respectively. The deviations of the content determination results of the two methods（except for chlorogenic acid ）were both not higher than 0.20％. CONCLUSIONS Established HPLC fingerprints are stable and feasible. Established QAMS method is accurate and rapid. HPLC fingerprint combined with QAMS can be used for the quality control for Yinhuang solution for inhalation ．
