Telmisartan affects proliferation, migration and apoptosis of non-small cell lung cancer cell A549 through the Wnt/β-catenin signaling pathway
- VernacularTitle:替米沙坦通过Wnt/β-catenin信号通路影响非小细胞肺癌A549细胞增殖、迁移和凋亡的研究
- Author:
Lingjie WANG
1
;
Donghua ZHAO
1
;
Zhangfeng HUANG
1
;
Mengjun LI
1
;
Pengfei GUO
1
;
Yongjie WANG
2
Author Information
1. Qingdao Medical College of Qingdao University, Qingdao, 266071, Shandong, P.R.China
2. Department of Thoracic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, 266071, Shandong, P.R.China
- Publication Type:Journal Article
- Keywords:
Telmisartan;
lung cancer;
apoptosis;
Wnt/β-catenin signaling pathway
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2022;29(01):100-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of telmisartan on the proliferation, migration and apoptosis of non-small cell lung cancer A549 and the mechanism of regulating Wnt signaling pathway. Methods Non-small cell lung cancer cell line A549 was cultured in vitro. Cell counting kit-8 (CCK-8) assay was used to detect the effect of telmisartan at different concentrations on the proliferative activity of A549 cells. The survival fraction of A549 treated with different concentrations of telmisartan was determined by colony-formation assay. The effect of telmisartan at different concentrations on the migration ability of A549 cells was examined in the wounding healing assay. Hoechst staining was used to detect the effects of telmisartan at different concentrations on the apoptosis of A549. Western bloting was used to detect the expressions of β-actin, proliferating cell nuclear antigen (PCNA), Bax, Bcl-2, Wnt-3a, Beta-catenin (β-catenin), serine protein kinase 3β (p-GSK-3β), glycogen synthase kinase-3β (GSK-3β) and c-myc. Results Different concentrations of telmisartan treatment inhibited the proliferation activity, colony-formation rate and migration of A549 cells, and reduced the expression of PCNA in a concentration-dependent manner. Telmisartan treatment promoted the apoptosis of A549 cells, significantly increased the expression of pro-apoptotic protein Bax and decreased the expression of anti-apoptotic protein Bcl-2. The expression levels of Wnt-3a, β-catenin, p-GSK-3β, and c-myc in A549 cells increased after treatment with telmisartan, while the expression levels of GSK-3β decreased. Conclusion Telmisartan may play a role in the proliferation, migration and apoptosis of non-small cell lung cancer A549 cells, and inhibiting the Wnt/β-catenin signaling pathway may be one of the mechanisms.
