Tissue-engineered cartilage nanoscaffolds prepared with collagen, hyaluronic acid and chondroitin sulfate
10.3760/cma.j.issn.1671-0290.2021.03.001
- VernacularTitle:胶原-透明质酸-硫酸软骨素构建组织工程软骨纳米支架的实验研究
- Author:
Zelong YANG
1
;
Ting JIANG
;
Zhu CHEN
;
Gang FENG
Author Information
1. 南充市中心医院（川北医学院第二临床学院）骨科，四川南充 637000
- Keywords:
Collagen;
Hyaluronic acid;
Chondroitin sulfates;
Electrostatic spinning;
Tissue-engineered cartilage
- From:Chinese Journal of Medical Aesthetics and Cosmetology
2021;27(3):161-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the possibility of constructing tissue-engineered cartilage three-dimensional nanoscaffolds with collagen Ⅱ (COLⅡ), hyaluronic acid (HA) and chondroitin sulfate (CS).Methods:The tissue-engineered cartilage scaffolds were prepared by electrospinning techniques with the mixture COLⅡ-HA-CS solvent, which dissolved by 3-trifluoroethanol-water. The surface topography was observed under electron microscope (SEM). And the diameter of nanofibers, the water absorption rate, contact angle and degradation rate were also detected. Generation 2 rabbit chondrocytes were seeded into the scaffold. The cell survival rate and proliferation were evaluated by Cell Counting Kit-8.Results:When the concentration range of electrospinning was 80-120 mg/ml and the mixing ratio of Col, HA and CS was 6-8∶1∶1-2, the tissue engineered cartilage nanoscaffolds could be successfully prepared. Their diameters were mainly distributed between 126.5±23.3 nm and 374.7±14.1 nm. The scaffolds had satisfactory hydrophilicity and degradability. The chondrocytes could well adhere and proliferate on the scaffold.Conclusions:The COLⅡ-HA-CS tissue-engineered cartilage nanoscaffolds have good physical and biological properties, which suggests its promising application in tissue-engineered cartilage.
