The standardization construction and quality management of colorectal neoplasm tissue sample database in the era of precision medic
10.3760/cma.j.cn113565-20200916-00298
- VernacularTitle:精准医疗时代下结直肠肿瘤组织样本库的标准化建设与质量管理
- Author:
Jing WANG
1
;
Chenyang GE
;
Jianfei FU
;
Wenxia XU
;
Jinlin DU
Author Information
1. 浙江大学医学院附属金华医院中心实验室 321000
- Keywords:
Colorectaln eoplasm;
Tissue biobank;
Quality control
- From:
Chinese Journal of Medical Science Research Management
2021;34(3):230-234
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a standard colorectal neoplasm tissue biobank with complete clinical information to provide high quality samples for fundamental and clinical research of colorectal neoplasm.Methods:Based on Affiliated Jinhua Hospital, Zhejiang University School of Medicine, to conduct structural design of colorectal neoplasm tissues, normal tissues and related information. Establish standard operating procedures from the collection and storage of tissue samples, standardize the entry of basic information, medical history, pathology and other relevant clinical information of the patients, and conduct random quality inspections on the pathological morphology and molecular level on a regular basis.Results:A tissue biobank of colorectal neoplasm was successfully constructed. During the establishment and improvement of this tissue biobank, standardized quality control was implemented during the whole-process including sample collection, warehousing, storage and delivery. According to the random sampling quality inspection, the RNA preservation effect was good, the rates of neoplasms in cancer tissue was >80%, and the clinical data of samples were complete.Conclusions:The preliminary construction of colorectal neoplasm tissue biobank not only improves the utilization value of tissue samples, but also provides a guarantee for realizing the bidirectional transformation of fundamental research and clinical application.
