Peripheral blood monoclonal B-cell and/or plasma cell detection by flow cytometry in screening and monitoring EBV-associated post-transplant lymphoproliferative disorders
10.3760/cma.j.cn114452-20210813-00501
- VernacularTitle:流式细胞术测定外周血单克隆B细胞和/或浆细胞在EBV相关移植后淋巴细胞增殖性疾病中的意义
- Author:
Xueying WU
1
;
Man CHEN
;
Aixian WANG
;
Junyi ZHEN
;
Meiwei GONG
;
Qing DU
;
Hui WANG
Author Information
1. 河北燕达陆道培医院检验科，廊坊 065201
- Keywords:
Flow cytometry;
Monoclonal cells;
Allogeneic hematopoietic stem cell transplantation;
Lymphoproliferative disease after transplantation
- From:
Chinese Journal of Laboratory Medicine
2021;44(12):1133-1137
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effectiveness of detecting MC B/P in PB by FCM for EBV+PTLD screening.Methods:481 patients with fever and large lymph nodes after allogenic hematopoietic stem cell transplantation (ALLO-SCT) in Ludaopi Hospital from 2018 to 2019 were retrospectively analyzed. The results of post-transplantation days, viral load (EBV, CMV) and MCB/P were detected. To evaluate the value of MC B/P in the diagnosis of PTLD by the sensitivity, specificity, positive predictive value and negative predictive. Logistic regression was used to analyze the clinical influencing factors of EBV-associated PTLD. The median fellow-up time was 449 days (range: 184 days to 700 days).Results:The diagnosis of PTLD was established in 51 patients. 55 patients who detecting MC B/P by FCM were positive. There were significant differences between the PTLD negative and positive groups in lymph node enlargement, age, EBV, CMV, monoclonal B cells, and monoclonal plasma cells ( P<0.05). Monoclonal plasma, monoclonal B and days after transplantation are important relationship with the diagnosis of PTLD, which have good diagnostic value for EBV-associated PTLD. Conclusion:FCM screening peripheral blood MC B/P has good diagnostic performance for EBV-associated PTLD. Monoclonal B, monoclonal plasma and the number of days of PTLD after transplantation were correlated with EBV-associated PTLD.
