Analysis of correlation between donor graft cells subsets and the progression of non-infectious pulmonary complications in children after allogeneic hematopoietic stem cell transplantation
10.3760/cma.j.cn114452-20210809-00490
- VernacularTitle:移植物淋巴细胞亚群表达与儿童异基因造血干细胞移植后非感染性肺部并发症的相关性分析
- Author:
Man CHEN
1
;
Wei ZHAO
;
Minjing FU
;
Aixian WANG
;
Xueying WU
;
Meiwei GONG
;
Junyi ZHEN
;
Guanlan YUE
;
Hui WANG
Author Information
1. 河北燕达陆道培医院检验科，廊坊 065201
- Keywords:
Air-Leak Syndrome;
Allogeneic hematopoietic stem cell transplantation;
Lymphocyte subsets;
Graft versus host disease
- From:
Chinese Journal of Laboratory Medicine
2021;44(12):1126-1132
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to analyze the counts (per kilogram of body weight) or percentages of transplanted lymphocyte subgroups in children with non-infectious pulmonary complications (NIPC) and air-leak syndrome (ALS) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and explore its significance in the progression of lung complications after transplantation.Methods:The patients with NIPC and ALS after allo-HSCT from January 2013 to December 2019 in Hebei Yanda Ludaopei Hospital were retrospectively studied and the influencing factors in the progress of NIPC after HSCT were statistically analyzed.Results:Of the 2026 children who received HSCT treatment, 59 patients (34 males and 25 females) developed NIPC, the probability was 2.9% (59/2 026), and the probability of combined ALS was 1.4% (28/206). The differences in the comparison between NIPC progressed to ALS group (ALS group) and failed to progress to ALS group (non-ALS group) in the patient′s age( P=0.028), disease condition before transplantation( P=0.022), NIPC onset time( P=0.004) were significant. The P values of the percentage of NKT-like cells in the bone marrow ( P=0.008) or peripheral stem cells ( P=0.003) accounted for the lymphocytes. CD4+CD25+dim cells in bone marrow ( P=0.029) or peripheral stem cells ( P=0.036) accounted for the CD4+lymphocytes and the ratio of CD4/CD8 in bone marrow( P=0.004) or peripheral stem cells ( P=0.020) were less than 0.05, which meant the differences in patients′ refusion cells were significant. In the binary logistic regression model, the percentage of bone marrow NKT-like cells to lymphocytes, the ratio of bone marrow CD4+/CD8+and the percentage of peripheral stem NK cells to lymphocytes were important risk factors for the progression of NIPC to ALS. The rest factors were excluded from the model (AUC=0.918, P<0.05). Conclusion:During allo-HSCT transplantation, a high proportion of NKT-like cell and NK cell levels, and a high CD4+/CD8+ratio in the infusion of donors with high immune tolerance have an important correlation with the progression of the NIPC.
