Preliminary study on the changes of Pole2 expression in the lymphocytes with aging as well as in the age-related diseases
10.3760/cma.j.cn114452-20210119-00047
- VernacularTitle:淋巴细胞Pole2增龄性表达变化及其在常见老年病患者中初步观察
- Author:
Chi ZHANG
1
;
Yuli HOU
;
Peichang WANG
Author Information
1. 首都医科大学宣武医院检验科，北京 100053
- Keywords:
Senescence;
Senile diseases;
Pole2;
DNA polymerase ε
- From:
Chinese Journal of Laboratory Medicine
2021;44(10):943-947
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the age-related changes of Pole2 expression levels in peripheral blood lymphocytes of healthy people, and analyze the differences of Pole2 expression levels in peripheral blood lymphocytes of four common senile disease patients between normal peers.Methods:Healthy people and patients in the physical examination center of Xuanwu Hospital of Capital Medical University from December 2018 to December 2019 were selected as the research objects, the mRNA and protein level of Pole2 in heathy individual as 20-29,30-39,40-49,50-59,60-69,70-79 y were checked by RT-PCR and Western blot,and the age-related curve was drew. The mRNA and protein expression levels of Pole2 were also detected in the peripheral blood lymphocytes of patients of DM,CHD,AD,CVD. And the deviation with healthy people of the same age was analyzed from one way ANOVA and repeated measurement were used to compare the mean of multiple groups.Results:The mRNA and protein levels of Pole2 increased in the lymphocytes of healthy people at 20-29 y and 50-59 y grouy, and decreased after 60-69 y and 70-79 y ( P<0.001). The Pole2 levels of mRNA and protein in the lymphocytes of patients of CHD,AD,DM and CVD cerebral atherosclerosis were significantly higher than that of healthy people of the same age. Moreover, the Pole2 level of lymphocytes of AD patients was significantly higher than that of other patient groups ( P<0.001). Conclusion:The expression level of Pole2 in peripheral blood lymphocytes increases with age before the age of 60 years old and decreases with age after 60 years old ;and the expression in four common senile diseases was higher than that in normal people.
