Research progress of POCT of nucleic acid based on CRISPR/Cas technology
10.3760/cma.j.cn114452-20210517-00309
- VernacularTitle:基于CRISPR/Cas技术的核酸即时检测研究进展
- Author:
Yaling CAO
1
;
Feng REN
Author Information
1. 首都医科大学附属北京佑安医院北京市肝病研究所，北京 100069
- Keywords:
Point-of-care testing;
CRISPR/Cas;
Nucleic acid testing;
Molecular diagnostic techniques
- From:
Chinese Journal of Laboratory Medicine
2021;44(9):864-867
- CountryChina
- Language:Chinese
-
Abstract:
In view of given that the current development trend of nucleic acid molecular detection technology is gradually shifting from clinical laboratory to field detection, there is an urgent need to establish a new nucleic acid diagnostic tool with high sensitivity, high specificity, great efficiency and convenience portability to meet the needs of clinical point-of-care testing(POCT). The detection method based on clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein (Cas) is a promising new nucleic acid detection method. In this method, the Cas effector protein would recognize highly specific nucleic acid sequences, and it can be used for POCT in combination with a highly sensitive small biosensor. This method can successfully achieves the high-efficiency and convenient detection characteristics while satisfying high sensitivity and high specificity. This article reviews the developments progress in the field of miniaturized analytical sensors related to CRISPR/Cas technology in recent years, and discusses the research prospects and challenges of related POCT detection.
