The single-cell proteomics methods based on liquid chromatography tandem mass spectrometry promote tumor diagnosis research
10.3760/cma.j.cn114452-20201230-00938
- VernacularTitle:基于液相色谱-串联质谱的单细胞蛋白质组学方法推动肿瘤诊断研究
- Author:
Yi HE
1
;
Xujun LIANG
;
Pengfei ZHANG
Author Information
1. 中南大学湘雅医院肿瘤科，国家卫健委肿瘤蛋白质组学重点实验室，长沙 410008
- Keywords:
Liquid chromatography tandem mass spectrometry;
Single cell;
Proteomics;
Sample preparation;
Microfluidics
- From:
Chinese Journal of Laboratory Medicine
2021;44(8):755-763
- CountryChina
- Language:Chinese
-
Abstract:
Single-cell proteome analysis can perform in-depth research on cell heterogeneity and further promote the development of precision medicine and tumor research. In recent years, mass spectrometry-based proteomics has made substantial progress, but proteomics analysis at the single-cell level still faces sensitivity challenges. At present, liquid chromatography tandem mass spectrometry has become the main analysis method of proteomics. Because of its high sensitivity, high throughput, and high stability, it has been widely used in the field of single cells. In recent years, the representative single-cell proteomics methods can be divided into three types: single-tube technology, microfluidic platform, and integrated processing platform according to different sample processing methods. Different kinds of data collection and analysis methods have their own advantages and disadvantages. If single-cell proteomics, still a laboratory study, can be applied in diagnostic practice as soon as possible, it will definitely promote the progress of precision medicine and oncology research.
