Evaluation of the performance of systems for whole blood C-reactive protein detection: a multi-center study
10.3760/cma.j.cn114452-20210219-00112
- VernacularTitle:全血C反应蛋白检测系统性能评价的多中心研究
- Author:
Juan CHENG
1
;
Huaiyuan LI
;
Haipeng LIU
;
Yuxin WANG
;
Jin XU
;
Shangyang SHE
;
Wei QU
;
Yidong WU
;
Guixia LI
;
Junmei YANG
;
Liya MO
;
Yun XIANG
;
Jiangwei KE
;
Liyue KUI
;
Lei ZHENG
;
Hongbing CHEN
;
Zhili YANG
;
Xin LYU
;
Hong ZHANG
;
Zhenhua TANG
;
Lijuan MA
;
Hongquan LUO
;
Xiangyang LI
;
Wenli ZHANG
;
Hui JIA
;
Huiming YE
;
Lijun TIAN
;
Qiuhui PAN
Author Information
1. 上海交通大学医学院附属上海儿童医学中心检验科，上海 200127
- Keywords:
C-reactive protein;
Detection system;
Evaluation of performance;
Multi-center study
- From:
Chinese Journal of Laboratory Medicine
2021;44(7):633-643
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the performance of the commonly used whole blood C-reactive protein (CRP) detection systems and give related recommendation on the performance requirements of detection systems.Methods:A total of 7 540 venous blood samples from 26 maternal, child and children′s hospitals were collected to conduct this multi-center study on the analytical performance of 5 commonly used whole blood CRP detection systems from March to April in 2019. The blank check, carryover, repeatability, intermediate precision, linearity, sample stability, influence of hematocrit/triglyceride/bilirubin, comparison with SIEMENS specific protein analyzer and trueness were evaluated. The 5 systems included BC-5390CRP autohematology analyzer, AstepPLUS specific protein analyzer, Ottoman-1000 Automated Specific Protein POCT Workstation, i-CHROMA Immunofluorometer equipment Reader and Orion QuikRead go detecting instrument. The 5 systems were labeled as a, b, c, d and e randomly.Results:Within the 5 systems, all values of blank check were less than 1.00 mg/L, the carryovers were lower than 1.00%. The repeatability of different ranges of CRP concentrations including 3.00-10.00, 10.00-30.00 and>30.00 mg/L were less than 10.00%, 6.00% and 5.00%, respectively, and the intermediate precision was less than 10.00%. The linearity correlation coefficients of the 5 systems were all above 0.975, while the slope was within 0.950-1.050. Whole blood samples were stable within 72 hours both at room temperature (18-25 ℃) and refrigerated temperature (2-8 ℃). The CRP results were rarely influenced by high triglyceride or bilirubin, except for the immmunoturbidimetric test based on microparticles coated with anti-human CRP F(ab) 2 fragments. When triglyceride was less than 15.46 mmol/L, the deviation of CRP was less than 10.00%. When bilirubin was less than 345.47 μmol/L, the deviation of CRP was less than 10.00%. CRP was more susceptible to Hct on the systems without Hct correction. The deviation of CRP between different Hct dilution concentration and 40% dilution concentration can reach as high as 67.48%. The correlation coefficients ( r) of 5 systems were all more than 0.975 in the range of 0-300.00 mg/L compared with Siemens specific protein analyzer. All systems passed the trueness verification using the samples with specified values of 12.89 and 30.60 mg/L. Conclusion:The performance of 5 systems can basically meet the clinical needs, but it is suggested that the whole blood CRP detection system without automatic Hct correction should be modified manually.
