Rapid identification of homology of carbapenem-resistant Escherichia coli by FTIR

VernacularTitle:采用傅里叶红外光谱技术快速分析碳青霉烯耐药大肠埃希菌同源性
Author: Jiayue LU ¹ ; Qiaoling SUN ; Ning DONG ; Congcong LIU ; Yu ZENG ; Yanyan HU ; Danxia GU ; Rong ZHANG
Author Information

1. 浙江大学第二附属医院检验科，杭州310000
Keywords: Escherichia coli; Carbapenems; Spectroscopy; Fourier transform infrared; Sequence homology
From: Chinese Journal of Laboratory Medicine 2021;44(6):503-508
CountryChina
Language:Chinese
Abstract: Objective:Evaluate the application of Fourier transform infrared spectroscopy in the identification of homology of carbapenem-resistant Escherichia coli(CREC). Methods:A total of 26 carbapenem-resistant Escherichia coli strains were isolated from 9 provinces in China in 2018. The 900-1 200 cm -1 was selected as a spectral region for the Euclidean distance calculating and average linkage clustering between all isolates.The single nucleotide polymorphism (SNP) was analyzed by whole genome sequencing (WGS). Results:Twenty-six CREC strains were divided into 14 infrared spectros copy(IR) types by FTIR. The same IR type belonged to the same sequence type type.Compared with cluster analysis based on WGS, the consistency of FTIR cluster analysis was 92.3% (24/26).Conclusions:FTIR presented excellent performance in identification of homology of CREC.Besides, with the advantages of simple operation and rapid acquisition of results, FTIR may be a useful tool in clinical labs.