Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae).
10.3347/kjp.2015.53.6.789
- Author:
Tiantian ZHANG
1
;
Xuejiao CUI
;
Jincheng ZHANG
;
Hui WANG
;
Meng WU
;
Hua ZENG
;
Yuanyuan CAO
;
Jingze LIU
;
Yonghong HU
Author Information
1. Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang, 050024, China. liujingze@mail.hebtu.edu.cn
- Publication Type:Brief Communication
- Keywords:
Dermacentor silvarum;
tick;
antigenic protein;
LC-MS/MS
- MeSH:
Animals;
Antigens/*chemistry/immunology;
Arthropod Proteins/*chemistry/immunology;
Electrophoresis, Polyacrylamide Gel;
Female;
Ixodidae/*chemistry/immunology;
Molecular Weight;
Rabbits;
Tandem Mass Spectrometry
- From:The Korean Journal of Parasitology
2015;53(6):789-793
- CountryRepublic of Korea
- Language:English
-
Abstract:
In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.
