Profile and gene functional analysis of gut microbiota in women with postmenopausal osteoporosis
10.3760/cma.j.cn112309-20210425-00134
- VernacularTitle:绝经后骨质疏松症患者肠道微生物组分及基因功能初步分析
- Author:
Jing LYU
1
;
Heping ZHAO
;
Yan YU
;
Yuhong ZENG
;
Kun DAI
;
Rong QIAO
;
Lei GUO
;
Jihan WANG
Author Information
1. 西安交通大学附属红会医院检验科 710054
- Keywords:
Postmenopausal osteoporosis;
Bone mineral density;
Gut microbiota;
Metagenomic sequencing
- From:
Chinese Journal of Microbiology and Immunology
2021;41(11):867-874
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the profile and gene functional changes of gut microbiota (GM) in women with postmenopausal osteoporosis (PMOP) in Northwest China, and the correlations between GM and bone mineral density (BMD).Methods:From November 2018 to October 2019, postmenopausal women were screened on their initial visits to our hospital, and 24 new osteoporosis (OP) patients, 30 new osteopenia patients and nine negative controls were recruited. Fecal samples were collected for GM DNA extraction, and Illumina platforms were used for high-throughput sequencing of 16S rRNA and metagenome. Species annotation, GM profile and gene functions were viewed and analyzed.Results:GM profiles were significatly different in different groups, and the LDA scores of Peptostreptococcaceae, Romboutsia, unidentified Clostridiales, Megamonas, Erysipelatoclostridium, Klebsiella and Erysipelatoclostridium ramosum were more than 3 in OP group. Metagenomic sequencing analysis indicated that gene numbers were positively correlated with BMD, and metabolism, carbohydrate metabolism, starch and sucrose metabolism and oxidative phosphorylation were negatively correlated with BMD. Receiver operating characteristic curve(ROC) showed that carbohydrate metabolism, starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism, oxidative phosphorylation, respectively, could identify OP with preferable sensitivity and specificity (areas under curve were 0.70, 0.72, 0.73 and 0.75, respectively). Conclusions:High-throughput sequencing had great potential for GM analysis of postmenopausal women with OP, providing evidence of the correlations between GM and BMD.
