Analysis of microRNAs and target genes associated with immune response to EVA71 and CVA16 infection in human respiratory epithelial cells
10.3760/cma.j.cn112309-20210409-00113
- VernacularTitle:EV71和CVA16感染人呼吸道上皮细胞后参与免疫应答相关的microRNA和靶基因的分析
- Author:
Xuelin ZHENG
1
,
2
;
Yajie HU
;
Hui LI
;
Xi JIANG
;
Shaozhong DONG
;
Jie SONG
Author Information
1. 中国医学科学院&
2. 北京协和医学院，医学生物学研究所，云南省重大传染病疫苗研发重点实验室，昆明 650118
- Keywords:
EV71;
CVA16;
miRNA;
Target gene
- From:
Chinese Journal of Microbiology and Immunology
2021;41(11):852-859
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze differentially expressed microRNAs (miRNAs) and target genes in human respiratory epithelial cells (16HBE) after enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) infection using high-throughput sequencing.Methods:TargetScan and miRDB databases were used to predict the target genes of miRNAs that were both up-regulated or down-regulated after EV71 and CVA16 infection. The genes that were both up-regulated and down-regulated were screened out. GO and pathway analysis of the target genes were conducted to screen immune-related target genes and their corresponding miRNAs. The target genes and their corresponding miRNAs that were up-regulated or down-regulated in both immune-related GO and pathway were further screened. Some miRNAs and their target genes were selected for qRT-PCR verification.Results:There were 598 target genes of up-regulated miRNAs and 1 311 target genes of down-regulated miRNAs and 62 target genes that might be up-regulated or down-regulated simultaneously were screened out. The number of up-regulated target genes involved in immune-related GO and pathway were 17 and 13, respectively, and the number of corresponding miRNAs were 15 and 17, respectively. There were 58 and 47 down-regulated target genes involved in immune-related GO and pathway, respectively, and the number of corresponding miRNAs were 30 and 42, respectively. Three up-regulated target genes were involved in both immune-related GO and pathway and regulated by four miRNAs. Nine down-regulated target genes were involved in both immune-related GO and pathway and regulated by 13 miRNAs.Conclusions:This study was conducive to elucidate the host-pathogen interaction after EV71 and CVA16 infection, and provided reference for studying the pathogenesis of hand, foot and mouth disease.
