Immunogenicity of MDCK cell-based quadrivalent influenza split vaccine with different adjuvants in mice
10.3760/cma.j.cn112309-20201102-00497
- VernacularTitle:不同佐剂细胞基质四价流感裂解疫苗的小鼠免疫效果评价
- Author:
Xuanxuan NIAN
1
;
Jing LIU
;
Tao DENG
;
Chuanshuo LYU
;
Qingmei ZHANG
;
Feixia PENG
;
Jiayou ZHANG
;
Xiaoming YANG
Author Information
1. 国家联合疫苗工程技术研究中心，武汉 430207
- Keywords:
Cell-based quadrivalent influenza split vaccine;
Adjuvant;
Stability;
Immunogenicity
- From:
Chinese Journal of Microbiology and Immunology
2021;41(9):711-718
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the immunogenicity of Madin-Darby canine kidney (MDCK) cell-based quadrivalent influenza split vaccine (MDCK-Va) combined with different adjuvants.Methods:Different doses of MDCK-Va and chicken embryo-based quadrivalent influenza split vaccine (egg-Va) were intramuscularly immunized BALB/c mice twice with an interval of three weeks. Serum samples were collected to detect antibody titers using hemagglutination inhibition (HI) assay. BALB/c mice were immunized with different doses of MDCK-Va combined with QS21, AddVax, PolyI∶C, CpG ODN 1826 and AddVax/PolyI∶C (Add/Poly), respectively. HI and microneutralization assays were used to detect antibody titers 21 d after the first and booster immunization. Spleen tissues were collected from the mice immunized with 10 μg MDCK-Va combined with the above adjuvants 5 d after the booster immunization to analyze spleen index and the types of spleen cells.Results:The immunoprotective effect of MDCK-Va was not inferior to that of egg-Va. MDCK-Va combined with each of the above adjuvants could induce higher HI antibody titer than MDCK-Va alone, especially the QS21/Va and Add/Poly/Va groups, and the differences were statistically significant. For H1N1 vaccine, the Pearson′s correlation coefficient ( r) between HI antibody and neutralizing antibody was 0.737-0.910, and for H3N2 subtype vaccine, the value of r was 0.839-0.947. Compared with the MDCK-Va group, the QS21/Va group showed significantly increased spleen index and decreased proportion of single lymphocytes. QS21 and Add/Poly were much better than other adjuvants in stimulating mouse splenic neutrophils and CD4/CD8 cells. Conclusions:Add/Poly had a stronger immune enhancement effect on MDCK-Va, suggesting that it was a potential adjuvant for MDCK-Va. The antibody titer detected by HI and MN assays had a strong positive correlation.
