Serotype distribution and phylogenetic analysis of virus complete genome from indigenous dengue patients in Guangzhou in 2019
10.3760/cma.j.cn112309-20210406-00108
- VernacularTitle:2019年广州本地登革热病例血清型分布及病毒全基因组进化分析
- Author:
Lingzhai ZHAO
1
;
Lu ZHANG
;
Wenxin HONG
;
Shuang QIU
;
Jiamin FENG
;
Xinghua TAN
;
Fuchun ZHANG
Author Information
1. 广州医科大学附属市八医院传染病研究所 510060
- Keywords:
Dengue fever;
Dengue virus;
Virus complete genome;
Phylogenetic analysis
- From:
Chinese Journal of Microbiology and Immunology
2021;41(9):698-703
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the serotype distribution and phylogenetic analysis of virus complete genome from indigenous dengue patients in Guangzhou in 2019 and provide evidence for the development of prevention and treatment strategies.Methods:Dengue virus serotypes of indigenous dengue cases in 2019 were detected using serotype specific fluorescent PCR kits. Complete genome in the culture was performed on Illumina platform. Phylogenetic analysis was conducted on complete genomes extracted from ViPR and the isolates from this study with MEGA7.0 software.Results:In 2019, three prevalent serotypes of dengue virus were found in Guangzhou, among which serotype 1 accounted for 80.35%, serotype 2 accounted for 12.97% and serotype 3 accounted for 6.68%. There were no significant differences in gender, age and severity among three serotypes. Phylogenetic analysis of virus complete genome showed that serotype 1 belonged to genotypeⅠand had two origins, which was close to the Cambodian strain; serotype 2 belonged to genotype cosmopolitan, which was close to the epidemic strain in Southeast Asia; serotype 3 belonged to genotypeⅢ, which was in the same branch as the Indian strain.Conclusions:The dengue epidemic was caused by dengue virus serotypes 1, 2 and 3 in Guangzhou in 2019. Each serotype belonged to a genotype.
