Genetic diversity and molecular epidemiology of Bordetella pertussis isolates
10.3760/cma.j.cn112309-20210311-00082
- VernacularTitle:百日咳鲍特菌的基因多样性及分子流行病学分析
- Author:
Juansheng ZHANG
1
;
Xiaoqiang WANG
;
Diqiang ZHANG
;
Hao LI
;
Xiaoguang WEI
;
Zengguo WANG
;
Ying LIU
;
Yuewan ZHAO
Author Information
1. 西安市疾病预防控制中心微生物检验科 710054
- Keywords:
Bordetella pertussis;
Genetic diversity;
Molecular epidemiology;
Multilocus antigen sequence typing (MAST);
Multilocus variable-number tandem repeat analy
- From:
Chinese Journal of Microbiology and Immunology
2021;41(9):687-691
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the genetic diversity and molecular epidemiology of Bordetella pertussis in Shaanxi province, and analyze the possible reasons of resurgence in this region. Methods:We characterized clinical isolates collected during 2012-2017 using multilocus antigen sequence typing (MAST) and multilocus variable-number tandem repeat analysis (MLVA).Results:The circulating strains and vaccine strains were different in molecular characteristics. The majority (95%) of the isolates were typed as prn1/ ptxP1/ ptxA1/ fim3-1/ fim2-1. In addition, eight MLVA types (MTs) and eight PFGE profiles were identified, respectively. MT195, MT55 and MT104 were dominant and MT195 continually increased annually. Conclusions:The genetic characteristics of the current strains in Shaanxi province were different from those of the vaccine strain. The evolution through genetic variation might be one of the reasons for the recurrence of pertussis in this region.
