Metabolomic alterations of human lung bronchial epithelial cells infected with human rhinovirus 1B
10.3760/cma.j.cn112309-20201228-00573
- VernacularTitle:人鼻病毒1B感染致人肺支气管上皮细胞代谢组改变的研究
- Author:
Meng SU
1
;
Dan LI
;
Pingping SUN
;
Chunyang WANG
;
Jiangli WANG
;
Hong WANG
;
Qing ZHANG
;
Luanying DU
;
Guangcheng XIE
Author Information
1. 承德医学院病原生物学教研室/病原防控研究室 067000
- Keywords:
Human rhinovirus 1B;
Human lung bronchial epithelial cells;
Metabolomics
- From:
Chinese Journal of Microbiology and Immunology
2021;41(7):545-549
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To research the metabolomic alterations of human lung bronchial epithelial cells infected with human rhinovirus 1B (HRV1B).Methods:Untargeted metabolomics was used to determine the metabolomic alterations in human lung bronchial epithelial cells (BEAS-2B) 6 h, 12 h and during the dynamic process (6 h∶12 h) after HRV1B infection.Results:A total of 93 differentially significant metabolites (DSMs) (47 DSMs were up-regulated and 46 DSMs were down-regulated) and 88 DSMs (37 DSMs were up-regulated and 51 DSMs were down-regulated) at post infection of HRV1B in BEAS-2B at 6 h or 12 h, respectively. A total of 30 DSMs (12 DSMs were up-regulated and 18 DSMs were down-regulated) in a dynamic process (6 h∶12 h) after HRV1B infection. Unknown metabolites took up most proportions. The trends of fatty acid, lipid, amino acid, nucleotide and carbohydrate were increased along with the prolonging of HRV1B infection. DSMs such as Diisononyl phthalate was co-detected DSMs among three groups.Conclusions:Metabolites such as fatty acid, lipid, amino acid, nucleotide and carbohydrate of BEAS-2B cells are changed induced by HRV1B infection.
