Establishment of allergic rhinitis mouse model sensitized by Artemisia annua and its immunological study
10.3760/cma.j.cn112309-20210531-00187
- VernacularTitle:黄花蒿花粉变应原致敏小鼠鼻炎模型建立及免疫学研究
- Author:
Yang YU
1
;
Ying ZHANG
;
Xu LU
;
Chenyu ZHOU
;
Lei YANG
;
Ming ZENG
;
Huiyun ZHANG
;
Miao XU
;
Shaoheng HE
Author Information
1. 锦州医科大学附属第一医院变态反应与临床免疫研究中心 121001
- Keywords:
Artemisia annua;
Allergic rhinitis;
Animal model;
Allergy
- From:
Chinese Journal of Microbiology and Immunology
2021;41(7):516-523
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the BALB/c mouse rhinitis model sensitized by Artemisia annua pollen allergen, and explore the humoral and cellular immune indicators that can be used for the evaluation of allergic reactions. Methods:Using BALB/c mice as experimental animals, using Artemisia annua pollen allergen extract as sensitizing protein, through different content of the main allergen Art a1 and different sensitization times, different immunization programs were set to immunize mice subcutaneously, One week and five weeks after the last immunization, Artemisia annua pollen allergen extract containing 50 μg/ml and 500 μg/ml Art a1 was used for nasal stimulation, once a day, for 1 week each time.Observe the allergic reaction of mice, detect the pathological changes of nasal tissues, determine the levels and dynamic changes of antigen-specific IgE, IgG1, IgG2a and other antibodies in the serum of each group of mice. and detect the changes in the number of antigen-specific IL-4, IL-5, IL-2, IFN-γ and other lymphocytes in the spleen of mice. Results:Sensitized mice showed obvious scratching and sneezing reactions after being stimulated by antigen; obvious allergic inflammation appeared in nasal tissue; The increase in serum level of Artemisia annua pollen-specific IgE antibody was significantly correlated with the challenge antigen; The antigen-specific IL-4 lymphocytes in the spleen of the sensitized mice were significantly increased, but the IFN-γ-specific lymphocytes did not change significantly. Conclusions:The successful establishment of a mouse model of Artemisia annua pollen allergen allergy is the first domestic use of ELISPOT technology to detect an increase in the number of antigen-specific IL-4 lymphocytes in Artemisia annua allergy mice, laying a foundation for the subsequent evaluation of the efficacy of preparations for desensitization treatment basis.
