Quinine oxidoreductase 1 inhibits the secretion of IL-8 in Mycoplasma pneumoniae lipid-associated membrane proteins stimulated the THP-1 monocyte line
10.3760/cma.j.cn112309-20200929-00458
- VernacularTitle:肺炎支原体脂质相关膜蛋白激活THP-1单核细胞系产生醌氧化还原酶1抑制IL-8的分泌
- Author:
Weiyan DING
1
;
Tianping TAN
;
Nan DING
;
Tingting LI
;
Lijun HUANG
;
Shuihong LI
;
Cuiming ZHU
Author Information
1. 南华大学衡阳医学院病原生物学研究所，特殊病原体防控湖南省重点实验室 421001
- Keywords:
Mycoplasma pneumoniae;
Lipid-associated membrane proteins;
Quinine oxidoreductase 1;
Nuclear factor erythroid 2-related factor 2;
Interleukin 8
- From:
Chinese Journal of Microbiology and Immunology
2021;41(6):460-465
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects and regulation mechanism of lipid-associated membrane proteins (LAMPs) derived from Mycoplasma pneumoniae( Mp) on the expression of quinine oxidoreductase 1 (NQO-1) in human monocyte cell line THP-1 cells, and to know the effect of NQO-1 to interleukin 8 secretion in LAMPs stimulated cells, so as to better understand the regulation mechanism upon Mp infection. Methods:Mp were cultivated and the precipitate was collected to extract LAMPs. The cytotoxicity of LAMPs to THP-1 cells was analyzed by using CCK8 test. THP-1 cells were cultured in vitro with different concentrations of LAMPs for different times, and the expression of NQO-1 protein was detected by Western blot. Nrf2 siRNA was used to investigate the role of Nrf2 in NQO-1 expression in LAMPs induced cells, and NQO-1 inhibitor Diminutol was performed to test whether they blocked interleukin 8 (IL-8) secretion when treated with LAMPs in THP-1 cells. Results:LAMPs extracted from Mp had no cytotoxicity to THP-1 cells. The expression of NQO-1 protein in LAMPs-stimulated THP-1 cells showed a dose-dependent and time-dependent manner. The production of NQO-1 protein reached peaks when treated with 5.0 μg/ml or 7.5 μg/ml of LAMPs for 12 h. Silencing of Nrf2 by siRNA significantly decreased NQO-1 production, and blocking NQO-1 by Dim increased the level of IL-8 in LAMPs-stimulated cells. Conclusions:LAMPs derived from Mp induced the expression of NQO-1 protein in THP-1 cells via Nrf2, and NQO-1 can inhibit IL-8 secretion in LAMPs stimulated monocytes.
