Research on the role of miR-125b-1-3p in rotavirus replication
10.3760/cma.j.cn112309-20210224-00063
- VernacularTitle:MiR-125b-1-3p在轮状病毒复制中的作用研究
- Author:
Lu NA
1
;
Panpan GENG
;
Yan ZHOU
;
Shibao YAO
;
Mei GUO
;
Hongjun LI
Author Information
1. 中国医学科学院，北京协和医学院医学生物学研究所分子生物室，昆明 650031
- Keywords:
Rotaviurs;
miRNA;
PI3K/Akt;
Virus replication;
Antiviral response
- From:
Chinese Journal of Microbiology and Immunology
2021;41(6):433-439
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role and regulatory mechanism of miR-125b-1-3p in rotavirus replication.Methods:MA104 cells were infected with rotavirus after upregulation and down-regulation of miR-125b-1-3p, respectively. The expression of miR-125b-1-3p and the copy number of rotavirus were analyzed by RT-PCR. The effect of miR-125b-1-3p on the protein expression of rotavirus was analyzed by immunofluorescence. The expression of related proteins involved in the regulation of miR-125b-1-3p was analyzed by Western blot analysis.Results:After rotavirus infection, the expression level of miR-125b-1-3p was significantly up-regulated, the copy number of VP7 and NSP3 gene of rotavirus decreased after up-regulation of miR-125b-1-3p, and the copy number of VP7 and NSP3 gene of rotavirus was significantly increased after down-regulation of miR-125b-1-3p.The fluorescence number of rotavirus protein decreased after upregulation of miR-125b-1-3p expression level, and increased after down-regulation of miR-125b-1-3p expression level. The activity of PI3K/Akt pathway was inhibited 16 h after rotavirus infection, and the up-regulation of miR-125b-1-3p could inhibit the activation of PI3K/Akt pathway.Conclusions:MiR-125b-1-3p inhibits rotavirus replication by regulating the PI3K/Akt pathway. These results provide an experimental basis for exploring the specific regulatory mechanism between miR-125b-1-3p and PI3K/Akt pathway, and provide a target for anti-infection therapy of rotavirus.
