Comparison of three methods to detect antibody response to SARS-CoV-2 vaccine
10.3760/cma.j.cn112309-20210427-00140
- VernacularTitle:新型冠状病毒疫苗免疫后抗体水平检测的3种实验方法比较
- Author:
Weixin CHEN
1
;
Meng CHEN
;
Shanshan ZHOU
;
Jiang WU
Author Information
1. 北京市疾病预防控制中心，北京市预防医学研究中心 100013
- Keywords:
SARS-CoV-2 vaccine;
SARS-CoV-2 IgG antibody;
Enzyme-linked immunosorbent assay;
Chemiluminescence method;
Neutralization test
- From:
Chinese Journal of Microbiology and Immunology
2021;41(6):423-426
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect IgG and neutralizing antibodies response to SARS-CoV-2 vaccine by comparing enzyme-linked immunosorbent assay (ELISA), commercial magnetic particle chemiluminescence assay(CLIA) and neutralization test(NT).Methods:ELISA, CLIA and NT were used to detect 143 healthy people before and after 28 days immunization with 2 doses of SARS-CoV-2 vaccine, and calculate the positive conversion rate, quantitative results and analysis the consistency of the three methods.Results:The positive conversion rate of SARS-CoV-2 vaccine antibody detected by ELISA, CLIA and NT were respectively 97.9%, 98.6% and 85.3%. The geometric mean of the highest dilution of the serum quantitatively detected by ELISA was 586.6; The mean of CLIA S/CO value was 11.26; The geometric mean titer of the NT was 7.6. The correlation coefficient between ELISA, CLIA and NT were respectively 0.69( P<0.01) and 0.65( P<0.01), and the correlation coefficient between ELISA and CLIA was 0.79( P<0.01). Conclusions:The three methods all detected high levels of antibodies response to SARS-CoV-2 vaccine immunization. ELISA and CLIA are more consistent to detect IgG antibody, and have a good correlation with the quantitative detection results of the NT.
