The effects of electro-acupuncture on learning, memory and stem cell differentiation in the hippocampus of rats modeling chronic cerebral ischemia
10.3760/cma.j.issn.0254-1424.2021.07.002
- VernacularTitle:电针对慢性脑缺血大鼠学习记忆及海马神经干细胞分化的影响
- Author:
Jurui WEI
1
;
Qi AI
;
Pei LIU
;
Wei XIA
;
Wei QIAN
;
Songtao JIANG
;
Junxia ZHENG
;
Songjie ZHU
;
Yun CHEN
Author Information
1. 浙江省临安区第一人民医院康复科，临安 311300
- Keywords:
Electro-acupuncture;
Cerebral Ischemia;
Neural stem cells;
Differentiation
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2021;43(7):582-587
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of electro-acupuncture (EA) on learning, memory and the differentiation of neural stem cells in the hippocampus in the presence of chronic cerebral ischemia.Methods:A total of 120 male Sprague-Dawley rats had chronic cerebral ischemia induced by bilateral ligation of the common carotid arteries. The model was successfully established in 104 of them, and they were randomly divided into a model group and an EA group, each of 52. The EA group was given 20 minutes of EA at acpoints Baihui and Dahui every day for 7 days, followed by an interval of 2 days. The current output was 1mA and the frequency was 15Hz. The model group was not given any intervention. One, 2, 4 and 6 weeks after the modelling, 6 rats from each group were injected with BrdU, and any proliferation and differentiation of neural stem cells was observed. The rats′ learning and memory were also evaluated using the Morris water maze, and neurogenesis of in the dentate gyrus was observed using BrdU+ NeuN and BrdU+ GFAP double-labelled immunofluorescence.Results:The learning and memory of the EA group were significantly better than those of the model group 2, 4 and 6 weeks after the modelling. After two weeks BrdU+ NeuN and BrdU+ GFAP-positive cells were found in the granule cell layer of the hippocampus, and compared with the model group, there were siginificantly more such neurons in the EA group. The gliocyte levels were not significantly different.Conclusion:Electro-acupuncture can improve learning and memory in the face of chronic cerebral ischemia by promoting the differentiation of neural stem cells.
