Evaluation of the quality of the IgM antibody capture ELISA diagnostic kit for the Japanese encephalitis virus produced by National Institute of Hygiene and Epidemiology
- Author:
Nga Thi Phan
;
Loan Phuong Do
;
Hoang Viet Nguyen
;
Trang Minh Bui
;
Thu Thi Hien Le
;
Tomohiko Takasaki
- Publication Type:Journal Article
- Keywords:
IgM antibody;
ELISA diagnostic kit;
Japanese encephalitis virus
- MeSH:
IgM antibody;
ELISA diagnostic kit;
Japanese encephalitis virus
- From:Journal of Preventive Medicine
2008;96(4):55-59
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: IgM antibody capture ELISA (MAC-ELISA) technique has been widely applied for Japanese Encephalitis Virus (JEV) diagnosis. So far rare internationally commercial kits are available. Thus, the international evaluation of the kit is required as per the recommendation of the WHO. Objectives: To evaluate the quality of the IgM antibody capture ELISA diagnostic kit for JEV produced by the Vietnam National Institute of Hygiene and Epidemiology (NIHE). Subjects and method: In this study, NIID kit was used as control to check the kit from NIHE. Both NIHE and NIID kits were used to detect JEV IgM among 38 serum and 6 CFS samples, which belongs to 5 sample groups (JE patients group, dengue patients group, other viral encephalitis patients group, Tick Born Encephalitis (TBE) patient group and healthy JE vaccinated donors group). Results: The detection of JEV IgM by NIHE kit was concurrent with the NIID kit. There is no positive with the JE in the groups of Dengue patients, TBE, other virus encephalitis patients and JE vaccinated donors. Conclusion: MAC-ELISA kit of NIHE can be used for different diagnosis of JEV and Dengue virus (both viruses are in Flavivirus genus), as well as other viruses caused by encephalitis.
