Puerarin attenuated ischemia/reperfusion injury an in vitro model through a repression of autophagy by HMGB1/TLR4/NF-κB axis
10.3760/cma.j.cn421203-20210326-00110
- VernacularTitle:葛根素通过HMGB1/TLR4/NF-κB轴抑制自噬减轻体外模型缺血再灌注损伤
- Author:
Yichen FAN
1
;
Jiayi YAN
;
Xueling LI
;
Ni LIU
;
Youhua ZHU
;
Mingxing SUI
;
Yifei ZHONG
Author Information
1. 上海中医药大学附属龙华医院 200032
- Keywords:
Kidney transplantation;
ischemia-reperfusion injury;
Puerarin
- From:
Chinese Journal of Organ Transplantation
2021;42(9):549-553
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the protective effect of puerarin on hypoxia/reoxygenation (H/R)-induced acute kidney injury(AKI)in vitro.Methods:HK-2 cells were treated with H/R for simulating ischemia reperfusion injury(IRI)in vivo. The experimental groups included control group, H/R treatment group(0/6/12/24 h), H/R 24 h + puerarin treatment group(puerarin, Pue), H/R 24 h + Pue+ 3-methyladenine(3-MA)treatment group and H/R 24 h+ 3-MA treatment group. Immunoblotting was employed for detecting the expression changes of autophagy-related proteins, CCK-8 for examining cell proliferation, electron microscopy for observing autophagosome formation and TUNEL for detecting apoptosis.Results:As compared with control group, the expression of LC3-II rose in H/R 24 h group, the expression of autophagy marker P62 declined, count of autophagosome increased, cell viability decreased and cellular inflammation occurred. Puerarin had similar effects to 3-MA. As compared with H/R 24 h group, puerarin could reverse the changes in the expression levels of LC3 and P62 induced by H/R( P<0.05). There were greater cell viability, reduced autophagosome count and lessened cell apoptosis( P<0.05). At the same time, protein expression levels of HMGB1, TLR4 and NF-κB dropped( P<0.05). Conclusions:Puerarin suppresses autophagy through HMGB1/TLR4/NF-κB axis for lessening ischemia-reperfusion injury an in vitro model.
