Establishing a diagnostic model for clinical almost tolerance of kidney allograft based upon plasma proteomics
10.3760/cma.j.cn421203-20200404-00107
- VernacularTitle:基于血浆蛋白质组学建立肾移植临床几乎耐受者诊断模型
- Author:
Honglai XU
1
;
Hongmin ZHOU
;
Min XIAO
;
Chao YANG
;
Yi GAO
;
Zhonghua CHEN
Author Information
1. 柳州市人民医院肝胆胰外科 545006
- Keywords:
Kidney transplantation;
Immunologic tolerance;
Proteomics
- From:
Chinese Journal of Organ Transplantation
2021;42(3):153-157
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore a new method of diagnosing and identifying renal transplantation clinical almost tolerance through a diagnostic model using plasma proteomics.Methods:From November 2011 to November 2012, plasma samples from 43 subjects were collected and divided into the groups of clinical almost tolerance(18 cases), rejection(12 cases)and healthy control(13 cases). Protemic analysis of plasma samples was performed by surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS). Differential mass spectrometry peaks were screened and diagnostic model was established by Biomarker Wizard and Biomarker Pattern software. Identification of mass spectrometric peaks of nodes in the diagnostic model was carried out by searching the databases of SWISS-PROT and TrEMBL using ExPASy's TagIdent tool.Results:A total of 21 differential proteins peaks were obtained( P<0.05). Diagnostic model was composed of five mass spectrum peaks of 2 565.15, 1 966.28, 6 674.78, 1 103.27, 1 716.69 and 1 966.28.The sensitivity, specificity and area under the ROC curve of model were 83.3%, 92.0% and 0.951 respectively for diagnosing clinical almost tolerance.Bioinformatic identification results of mass spectrometric peaks of nodes in model were proteins of ANFB, MCH, TFF1, PDYN and PSME3. Conclusions:Establishing a diagnostic mode by plasma proteomics may be effectively employed for diagnosing clinical almost tolerance in kidney transplant.
