Effect of specific small interfering RNA against KLF11 expression on proliferation and apoptosis of human gastric cancer cell line BGC823 via Wnt/β-catenin signaling pathway
10.3760/cma.j.cn113855-20210309-00146
- VernacularTitle:沉默KLF11表达通过Wnt/β-catenin信号通路对胃癌细胞BGC823增殖和凋亡的影响
- Author:
Qingwei LIU
1
;
Yong LI
;
Bibo TAN
;
Liqiao FAN
;
Qun ZHAO
;
Qiang JI
;
Zhaoxing LI
;
Ming TAN
;
Yijie ZHAO
;
Xinyu YUAN
Author Information
1. 河北医科大学第四医院外三科，石家庄 050010
- Keywords:
Stomach neoplasms;
Transcription factors;
Cell proliferation;
Apoptosis.;
Cell cycle
- From:
Chinese Journal of General Surgery
2021;36(8):616-620
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of KLF11 in gastric cancer tissues and cell lines as well as its effect on proliferation and apoptosis of human gastric cancer cells BGC823.Methods:Sixty pairs gastric cancer tissues and corresponding adjacent tissues were collected. The expression of KLF11 mRNA in gastric cancer tissues and their adjacent tissues was detected by RT-PCR. The expression of KLF11 was detected in gastric cancer cells. KLF11 expression was silenced. The proliferation of cells were detected by using MTT assay. Flow cytometry was used to detect the cell cycle and cell apoptosis rate. Western bloting was used to examine the changes of concentration of proteins associated with cell cycle,cell apoptosis and Wnt/β-catenin signaling pathway related proteins. The activity of Caspase3 enzyme was detected by spectrophotometry.Results:The relative expression of KLF11 mRNA in gastric carcinoma tissues was significantly higher than that of the adjacent tissues ( t=11.38, P<0.05). Its expression was related to tumor size, depth of invasion, lymph node metastasis and TNM clinical stage (all P<0.05). The proliferation of BGC823 cells was significantly suppressed after KLF11 silencing ( F=19.56, P<0.05), and the cell cycle was arrested in G 0/G 1 phase [(41.40%±0.98%) vs. (66.53%±1.01%), F=32.69, P<0.05]. Meanwhile, the apoptosis rate was significantly increased by KFL11 silencing [(41.44%±1.59%) vs. (15.42%±0.86%), F=35.35, P<0.05]. The results of Western blotting revealed that the expression of Bax and Cleaved Caspase3 was significantly increased ( F=23.33, 33.63; both P<0.05), wheras that of β-catenin, Bcl-2, CyclinD1and CyclinE was significantly reduced ( F=22.21, 16.24, 26.75, 33.42; all P<0.05). The activity of Caspase3 enzyme was enhanced ( F=16.56, P<0.05). Conclusion:KLF11 was highly-expressed in gastric cancer tissues and cells, KFL11 silencing could inhibit gastric cancer cells proliferation and induce cell apoptosis via Wnt/β-catenin signaling pathway.
