Berberine reduces the expression of adipogenic enzymes and inflammatory molecules of 3T3-L1 adipocyte.
- Author:
Bong Hyuk CHOI
1
;
In Sook AHN
;
Yu Hee KIM
;
Ji Won PARK
;
So Young LEE
;
Chang Kee HYUN
;
Myoung Soo DO
Author Information
1. School of Life and Food Sciences, Handong Global University, Pohang 791-708, Korea. msdo@handong.edu
- Publication Type:Original Article
- Keywords:
adipocytes;
berberine;
inflammation;
lipoprotein lipase;
PPAR-gamma;
tumor necrosis factor-alpha
- MeSH:
RNA, Messenger/genetics/metabolism;
Mice;
Leptin/secretion;
Inflammation Mediators/*metabolism;
Inflammation/genetics;
Glycerol/metabolism;
Gene Expression Regulation/*drug effects;
Cytokines/genetics;
Berberine/*pharmacology;
Animals;
Adipogenesis/drug effects/genetics;
Adipocytes/*drug effects/enzymology/*metabolism/secretion;
3T3-L1 Cells
- From:Experimental & Molecular Medicine
2006;38(6):599-605
- CountryRepublic of Korea
- Language:English
-
Abstract:
Berberine (BBR), an isoquinoline alkaloid, has a wide range of pharmacological effects, yet its exact mechanism is unknown. In order to understand the anti-adipogenic effect of BBR, we studied the change of expression of several adipogenic enzymes of 3T3-L1 cells by BBR treatment. First, we measured the change of leptin and glycerol in the medium of 3T3-L1 cells treated with 1 micrometer, 5 micrometer and 10 micrometer concentrations of BBR. We also measured the changes of adipogenic and lipolytic factors of 3T3-L1. In 3T3-L1 cells, both leptin and adipogenic factors (SREBP-1c, C/EBP-alpha, PPAR-gamma, fatty acid synthase, acetyl-CoA carboxylase, acyl-CoA synthase and lipoprotein lipase) were reduced by BBR treatment. Glycerol secretion was increased, whereas expression of lipolytic enzymes (hormone-sensitive lipase and perilipin) mRNA was slightly decreased. Next, we measured the change of inflammation markers of 3T3-L1 cells by BBR treatment. This resulted in the down-regulation of mRNA level of inflammation markers such as TNF-alpha, IL-6, C- reactive protein and haptoglobin. Taken together, our data shows that BBR has both anti-adipogenic and anti-inflammatory effects on 3T3-L1 adipocytes, and the anti-adipogenic effect seems to be due to the down-regulation of adipogenic enzymes and transcription factors.
