Effect of miR-425-5p on GLP-1 secretion in intestinal L cells induced by lipopolysaccharide and its mechanism
10.3760/cma.j.cn311282-20200928-00661
- VernacularTitle:miR-425-5p对脂多糖诱导的肠道L细胞GLP-1分泌的影响及机制研究
- Author:
Jiao WANG
1
;
Lirui WEI
;
Fengjiao HUANG
;
Xuenan ZHAO
;
Feng GUO
;
Lina WU
;
Yanling LIU
;
Guijun QIN
Author Information
1. 郑州大学第一附属医院内分泌与代谢病科 450052
- Keywords:
miR-425-5p;
Lipopolysaccharide;
Phosphatase and tensin homology;
β-catenin;
Intestinal L cells
- From:
Chinese Journal of Endocrinology and Metabolism
2021;37(7):646-652
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of miR-425-5p on glucagon-like peptide-1(GLP-1) secretion in intestinal L cells induced by lipopolysaccharide(LPS), and to explore its mechanism.Methods:GLUTag cells of intestinal L cell line were incubated with LPS to determine the levels of miR-425-5p and GLP-1. Cell viability was determined by MTT assay, and cell apoptosis was detected by flow cytometry. Quantitative real time-PCR and western blot were performed to determine the expressions of miR-425-5p, phosphatase and tensin homology(PTEN), proglucagon, and GLP-1. Activity of Wnt/β-catenin signaling pathway was determined by detecting TOP/FOP ratio. Interaction among miR-425-5p, PTEN, and β-catenin was analyzed using luciferase activity assay and chromatin immunoprecipitation(ChIP)assay.Results:In GLUTag cells, with the elevation of LPS concentration, the expression of miR-425-5p and the apoptosis rate were increased, while the level of active GLP-1 and the cell viability were decreased. MiR-425-5p was involved in the regulation of LPS on GLP-1 secretion and intestinal L cell viability. Inhibition of miR-425-5p reduced the mRNA expression of proglucagon and the TOP/FOP ratio, increased PTEN protein level, and inhibited cell viability. In LPS-treated GLUTag cells, miR-425-5p increased the level of β-catenin by targeting PTEN, and β-catenin acted as a cis-acting element to induce the transcription of proglucagon and promote the secretion of GLP-1.Conclusion:In LPS-induced intestinal L cells, miR-425-5p promotes the expression of GLP-1 by targeting PTEN to modulate β-catenin.
