Effects of different anesthetics on concentrations of Aβ and tau protein in cerebrospinal fluid of sleep deprived rats
10.3760/cma.j.cn131073.20210306.01015
- VernacularTitle:不同麻醉药对睡眠剥夺大鼠脑脊液Aβ和tau蛋白浓度的影响
- Author:
Yali JIAO
1
;
Yaying XIE
;
Jianshe YU
Author Information
1. 内蒙古自治区妇幼保健院麻醉科，呼和浩特 010010
- Keywords:
Anesthetics;
Sleep deprivation;
Cerebrospinal fluid;
Amyloid;
tau proteins
- From:
Chinese Journal of Anesthesiology
2021;41(10):1218-1221
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of propofol, dexmedetomidine and ketamine on oncentrations of β-amyloid peptide (Aβ) and tau in cerebrospinal fluid (CSF) of sleep-deprived rats.Methods:Forty SPF healthy male Sprague-Dawley rats, aged 3-4 months, weighing 230-280 g, were divided into 5 groups ( n=8 each) using a random number table method: control group (group C), sleep deprivation group (SD), propofol group (group P), dexmedetomidine group (group D) and ketamine group (group K). The sleep deprivation was induced using the improved multi-platform sleep deprivation model.Propofol 100 mg/kg, dexmedetomidine 100 mg/kg and ketamine 80 mg/kg were intraperitoneally injected at 72 h of sleep deprivation to maintain anesthesia for 3 h in P, D and K groups, respectively.Group C entered the large platform for 72 h free activity.The CSF was collected at 3 h of anesthesia for measurement of concentrations of Aβ and tau protein by enzyme-linked immunosorbent assay. Results:The concentrations of Aβ and tau protein in CSF were significantly higher in SD, P, K and D groups than in group C ( P<0.05). Compared with group SD, the concentrations of Aβ and tau protein in CSF were significantly increased in P and K groups, and the concentrations of Aβ and tau protein in CSF were significantly decreased in group D ( P<0.05). Conclusion:Dexmedetomidine can decrease the the concentrations of Aβ and tau protein in CSF of sleep deprived rats, while propofol and ketamine lead to the opposite effect.
