Changes in glucose metabolism in prefrontal cortex during long-term cognitive dysfunction induced by neuropathic pain in developing rats
10.3760/cma.j.cn131073.20210709.00920
- VernacularTitle:发育期大鼠神经病理性痛诱发远期认知功能障碍时大脑前额叶皮层葡萄糖代谢的变化
- Author:
Yuanyuan FANG
1
;
Lirong WANG
;
Jinpiao ZHU
;
Yifei HUANG
;
Jie WANG
;
Chang CHEN
;
Yanlin WANG
;
Zongze ZHANG
Author Information
1. 武汉大学中南医院麻醉科 430071
- Keywords:
Neuralgia;
Cognitive dysfunction;
Prefrontal cortex;
Glucose metabolism
- From:
Chinese Journal of Anesthesiology
2021;41(9):1124-1127
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the changes in glucose metabolism in the prefrontal cortex during long-term cognitive dysfunction induced by neuropathic pain in developing rats.Methods:SPF healthy male Sprague-Dawley rats, aged 4 weeks, weighing 80-100 g, were used in this study.The model of neuropathic pain was established by using spared nerve injury in anesthetized rats.The mechanical paw withdrawal threshold (MWT) was measured at 1 day before establishing the model (T 0) and 1, 3, 7, 14, 28, 42 and 56 days after establishing the model (T 1-7). According to the results of MWT compared between T 5 and T 0, the rats were divided into neuropathic pain group (group NP) and non-neuropathic pain group (group NNP). Open field test and novel object recognition test were performed at T 7 to assess anxiety-like behavior and cognitive function.Positron emission tomography/computed tomography imaging was performed to determine the standard uptake value of 18F-fluorodeoxyglucose in the prefrontal cortex.Then the rats were sacrificed, and prefrontal cortex was removed for determination of the expression of glucose transporter 3 using Western blot and immunofluorescence. Results:Compared with the baseline at T 0, the MWT at T 1-2 in group NNP and at T 1-7 in group NP were significantly decreased ( P<0.05). Compared with group NNP, the MWT at T 1-7 were significantly decreased, the time of staying at the central region at T 7 was shortened, the percentage of time for exploring the novel object was decreased, the percentage of novel object exploration was decreased, the standard uptake value of 18F-fluorodeoxyglucose in prefrontal cortex was decreased, and the expression of glucose transporter 3 in prefrontal cortex was down-regulated in group NP ( P<0.05). Conclusion:Long-term cognitive dysfunction induced by neuropathic pain may be related to decreased glucose metabolism in the prefrontal cortex of the developing rats.
