Effects of urinary exosomal miR-150-5p on renal senescence in different age groups
10.3760/cma.j.issn.0254-9026.2021.11.010
- VernacularTitle:不同年龄段人群尿液外泌体中miR-150-5p对肾脏衰老的影响
- Author:
Shan WANG
1
;
Qing MA
;
Huayu YANG
;
Lan YAO
;
Ning DING
Author Information
1. 首都医科大学附属北京友谊医院老年医学科 100050
- Keywords:
Renal;
Aging;
Renal epithelial mesenchymal transition;
Urine;
Exosome;
Minimal RNA;
MiR-150-5p
- From:
Chinese Journal of Geriatrics
2021;40(11):1386-1391
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of urinary exosomal miR-150-5p in different age groups and its relationship with kidney aging.Methods:Seventy-six subjects were recruited at the geriatrics department of Beijing Friendship Hospital from January 2014 to December 2016, with 15 in the young group, 35 in the middle-aged group, and 26 in the elderly group.We detected the expression of urinary exosomal miR-150-5p by real-time PCR from urine samples of different age groups and used Logistic regression analysis to evaluate the correlation of different levels of miR-150-5p with age, gender, history of hypertension, history of coronary heart disease, and hyperlipidemia.Additionally after overexpression and knockdown of miR-150-5p in human renal tubular cells of the HK2 cell line, we analyzed the expression levels of epithelial-to-mesenchymal transition(EMT)-related proteins by Western blotting.Results:Logistic regression analysis showed that age was closely related to estimated urinary exosomal miR-150-5p levels( P<0.05), and estimated urinary exosomal miR-150-5p levels in the elderly group(1.33±0.41)were significantly higher than those in the young group(0.88±0.40)( P<0.05). In addition, estimated urinary exosomal miR-150-5p levels became inversely proportional to the glomerular filtration rate as age increased. In vitro experiments showed that the expression levels of ZO-1 and E-cadherin in epithelial cells were slightly decreased after knockdown of miR-150-5p in renal tubular epithelial cells of the HK-2 cell line. Conclusions:The expression of miR-150-5p in urinary exosomes may be slightly increased in the elderly.It could inhibit the occurrence of renal EMT, and its expression is correlated with renal function.Mir-150-5p may also affect the expression of adhesion molecule-related proteins in HK-2 cells.MiR-150-5p in urinary exosomes is expected to become one of the genetic markers associated with renal aging.
