Effect of bile on intestinal alkaline phosphatase in intestinal mucosa
10.3760/cma.j.cn113884-20200724-00397
- VernacularTitle:胆汁对肠黏膜中肠碱性磷酸酶表达的影响
- Author:
Jun WAN
1
;
Baiyu YAO
;
Chong LIU
;
Jingni HE
;
Yao ZHOU
;
Yang SHI
;
Zhong TIAN
Author Information
1. 中国医科大学附属盛京医院普通外科，沈阳 110000
- Keywords:
Alkaline phosphatase;
Intestines;
Bile;
Jaundice, obstructive;
Caco-2 cell
- From:
Chinese Journal of Hepatobiliary Surgery
2021;27(6):449-453
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of intestinal alkaline phosphatase (IAP) in intestinal mucosa with bile deficiency and the effect of bile on the expression of IAP in intestinal epithelial Caco-2 cell model.Methods:Thirty healthy male SD rats were randomly divided into control group (Ctrl, n=10), external drainage group (ED, n=10) and obstructive jaundice group (OJ, n=10). Ileum specimens were collected on the 7th day after modeling. Western blot and immunohistochemical staining were used to determine the expression of IAP in rat intestinal mucosa. Different concentrations of human bile were used to treat on Caco-2 cells, and Western blot was used to detect the changes in IAP expression in Caco-2 cells. Results:Rat models were successfully established. The expression level of IAP in the intestinal mucosa of ED group [(9.19±1.67)%] was significantly lower than that of the Ctrl group [(15.09±0.61)%, P<0.05]; the expression of IAP in the intestinal mucosa of OJ group [(6.86±1.07)%] was significantly lower than that of the Ctrl group ( P<0.05). Through in vitro cell experiments, expression of IAP in Caco-2 cells was increased in a time and dose-dependent manner when treated with human bile. Conclusions:Bile deficiency in the intestine can cause inhibition of IAP in the intestinal mucosa. Bile can promote the expression of IAP in intestinal mucosal epithelial cells.
