miR-205-5p/E2F1 signal axis is involved in the regulation of radiosensitivity of glioma cells through suppressing the classical Wnt/β-catenin signaling pathway
10.3760/cma.j.cn113030-20200708-00352
- VernacularTitle:miR-205-5p/E2F1信号抑制经典Wnt/β蛋白通路调控脑胶质瘤细胞放射敏感性
- Author:
Youdong ZHOU
1
;
Ran LUO
;
Yanting LIU
;
Yuanxun DONG
;
Jinyang MA
;
Huojun HU
;
Xuguang WANG
;
Jinman GUO
;
Song HUANG
;
Gao YUAN
;
Changtao FU
;
Lei WANG
;
Yan GAO
Author Information
1. 三峡大学第一临床学院宜昌市中心人民医院神经外科 443000
- Keywords:
E2F1 transcription factor;
miR-205-5p gene;
Radiosensitivity;
Glioma cell line
- From:
Chinese Journal of Radiation Oncology
2021;30(11):1188-1194
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of miR-205-5p/E2F1 signal axis in regulating the glioma U251, U87 radiotherapy resistance.Methods:X-ray gradual ascending and intermittent induction method was used to irradiate the glioma U251 cells to establish U251/TR, U87/TR radiation-resistant cell lines. Then, the morphology, migration, invasion and proliferation abilities of cells (U251/TR, U87/TR radiation-resistant cells and U251, U87 radiation-sensitive cells) were analyzed. Luciferase gene detection system and point mutation technique were employed to analyze the mechanism of miR-205-5p and E2F1 gene activity on U251 and U87 radiation-resistant cell lines.Results:Compared with the radiation-sensitive U251 cells, the radiation-resistant cells U251/TR, U87/TR showed increased proliferation activity, enhanced migration and invasion abilities and decreased apoptosis under X-ray irradiation. miR-205-5p mimics transfection could down-regulate the expression of E2F1 factor in U251/TR cells, inhibit cell proliferation, invasion and migration and increase the radiosensitivity of U251/TR cells. miR-205-5p mimics transfection combined with with E2F1 down-regulation exerted anti-tumor effect and decreased cell tolerance by suppressing the Wnt/β-catenin signaling pathway activity.Conclusions:The glioma radiation-resistant cell line U251/TR, U87/TR can be established by X-ray gradual ascending and intermittent induction method. The miR-205-5p/E2F1 signal axis exerts tumor-suppressing effect through the classical Wnt/β-catenin signaling pathway, which can be used as an therapeutic target to increase the radiosensitivity of glioma.
