Role of miR-133a in radiation-induced cardiac injury in mice
10.3760/cma.j.cn113030-20200407-00156
- VernacularTitle:miR-133a在小鼠放射性心脏损伤中的作用
- Author:
Rui YAN
1
;
Jianbo SONG
;
Min GUO
;
Honghong CAI
;
Xianhai XU
;
Yarong ZHANG
;
Yang YU
;
Sijin LI
Author Information
1. 山西医科大学第一医院核医学科，太原 030001
- Keywords:
miR-133a gene;
Radiation-induced heart disease;
Myocardial fibrosis;
Mouse
- From:
Chinese Journal of Radiation Oncology
2021;30(10):1078-1083
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulatory role of microRNA in radiation-induced heart disease (RIHD) in mice and provide a new strategy for its treatment.Methods:Based on the Gene Expression Omnibus database (GSE147241), which includes normal heart tissue and irradiation heart tissue, we conducted bioinformatics research and analysis to determine the differentially-expressed genes. Then, thirty male C57/BL6 mice were randomly divided into the control group, irradiation group and miR-133a overexpression intervention group. The heart received single dose of X-ray 20 Gy in the irradiation group and miR-133a overexpression intervention group, but not in the control group, and then fed for 16 weeks. Cardiac function was assessed by echocardiography. Myocardial fibrosis was detected by Masson staining. The expression levels of miR-133a, CTGF, COL-1 and COL-3 mRNA were detected by qRT-PCR. The expression levels of CTGF, COL-1 and COL-3 proteins were detected by western blot.Results:miR-133a was the differentially-expressed gene between the irradiation and control groups. Overexpression of miR-133a could mitigate the decrease in cardiac function and increase in myocardial collagen content ( P<0.01). Meantime, overexpression of miR-133a could down-regulate the expression levels of CTGF, COL-1, COL-3 mRNA and protein ( P<0.01). Conclusions:Radiation increases the synthesis of collagen and leads to myocardial fibrosis remodeling. Overexpression of miR-133a can alleviate the radiation-induced myocardial fibrosis.
