Effect of resveratrol combined with radiotherapy on cervical cancer Hela cell growth and migration by regulating PI3K/Akt/mTOR signaling pathway
10.3760/cma.j.cn113030-20200525-00272
- VernacularTitle:白藜芦醇联合γ射线调控PI3K/Akt/mTOR信号通路对宫颈癌Hela细胞的影响
- Author:
Jianfu ZHANG
1
;
Yunjun ZHANG
;
Bengui JIANG
;
Haibing MEI
;
Huizhen ZHONG
Author Information
1. 宁波市妇女儿童医院药剂科 315012
- Keywords:
Resveratrol;
γ-ray;
PI3K/Akt/mTOR signaling pathway;
Hela cell line
- From:
Chinese Journal of Radiation Oncology
2021;30(9):956-960
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of resveratrol combined with γ-ray irradiation on the biological behavior of cervical cancer cells, and to explore its possible mechanism.Methods:The proliferation of cell populations after different concentrations of resveratrol solution±γ-ray irradiation was detected by CCK-8 assay. Scratch test and Transwell chamber test were used to detect cell migration and invasion. Flow cytometry and Annexin V-FITC/PI double staining were employed to assess cell apoptosis. Western blot was performed to measure the expression levels of PI3K, Akt, p-Akt, mTOR and p-mTOR proteins.Results:Compared with the normal control (NC) group, the resveratrol group±γ-ray irradiation could inhibit the proliferation, migration, and invasion and promote cell apoptosis of human cervical cancer Hela cells, and the combined effect was more obvious. Compared with the NC group, resveratrol and γ-ray irradiation could significantly down-regulate the expression levels of Bcl-2, PI3K, p-Akt and p-mTOR proteins, up-regulate the expression level of Bax protein, but did not significantly alter the expression levels of Akt and mTOR proteins in human cervic1 255al cancer Hela cells.Conclusions:Resveratrol combined with γ-ray irradiation can dramatically inhibit the proliferation, migration, invasion, and promote the apoptosis of cervical cancer Hela cells. The mechanism may be related to the inhibition of the PI3K/Akt/mTOR signaling pathway and down-regulating the expression levels of downstream related proteins.
