Silencing lncRNA UCA1 affects radiosensitivity of glioma cells by up-regulating miR-873-5p expression
10.3760/cma.j.cn113030-20191127-00490
- VernacularTitle:沉默lncRNA UCA1通过上调miR-873-5p表达对胶质瘤细胞放射敏感性影响
- Author:
Jinjin YUAN
1
;
Zongwen LIU
;
Rui SONG
;
Junqi LIU
;
Ruitai FAN
Author Information
1. 郑州大学第二附属医院肿瘤放疗科 450014
- Keywords:
lncRNA UCA1;
miR-873-5p;
Glioma cell line;
Xenografts of nude mouse;
Radiosensitivity
- From:
Chinese Journal of Radiation Oncology
2021;30(8):846-852
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of lncRNA UCA1 on the radiosensitivity of in vitro cultured glioma cell lines SHG-44, U87 and U251 by regulating the miR-873-5p expression. Methods:The survival of glioma cells SHG-44, U87 and U251 treated with different radiation intensities (0, 2, 4, 6 and 8 Gy) was detected by colony formation assay. The expression levels of UCA1 in glioma cells SHG-44, U87 and U251 were measured by qRT-PCR. The radiation-resistant glioma cells U87 and U251 were selected for subsequent study. After silencing UCA1 expression and/or over-expressing miR-873-5p, the cell survival rate was detected by colony formation assay, and the cell apoptosis rate was determined by flow cytometry. The dual luciferase reporter gene assay and qRT-PCR were employed to verify the targeting relationship between UCA1 and miR-873-5p.Results:UCA1 was up-regulated in the radiation-resistant U87 and U251 cells. Silencing UCA1 or over-expressing miR-873-5p inhibited the survival of U87 and U251 cells, and promoted the cell apoptosis induced by radiation exposure. miR-873-5p was a target gene of UCA1, and UCA1 negatively regulated the expression of miR-873-5p. The inhibition of miR-873-5p could reverse the effect of silencing UCA1 on the radiosensitivity of glioma cells. Silencing UCA1 increased the inhibitory effect of radiation on the glioma cell U251 xenografts.Conclusion:Silencing UCA1 inhibits the survival of glioma cells and promotes the cell apoptosis by up-regulating the expression of miR-873-5p, thereby increasing the radiosensitivity of glioma cells.
