Correlation between changes of oral bacterial flora and radiation-induced oral mucositis during radiotherapy for head and neck
10.3760/cma.j.cn113030-20210307-00091
- VernacularTitle:头颈部放疗期间口腔细菌菌群变化及其与放射性口腔黏膜炎相关性研究
- Author:
Zekai SHU
1
;
Baikui WANG
;
Lei WANG
;
Ming CHEN
;
Weifen LI
;
Yuanyuan CHEN
Author Information
1. 浙江中医药大学第二临床医学院，杭州 310053
- Keywords:
Radiation-induced oral mucositis;
Oral bacterial flora;
High-throughput sequencing;
Nasopharyngeal neoplasm/radiotherapy;
Mouse
- From:
Chinese Journal of Radiation Oncology
2021;30(7):665-670
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the correlation between the changes of oral bacterial flora during head and neck radiotherapy and radiation-induced oral mucositis (ROM).Methods:The oral bacterial samples of patients with nasopharyngeal carcinoma and accompanying family members were obtained before and at the end of radiotherapy and subjected to high-throughput sequencing. C57BL/6 mice were used to establish the ROM models. On the 9 th day after radiotherapy, oral bacterial samples were collected in the radiotherapy group and the negative control group. On the 3 rd, 5 th, 7 th, and 9 th days post-radiotherapy, the tongue tissues were obtained from another batch of mice in the negative control and radiotherapy groups. Inflammatory factors were detected with PCR and HE staining was performed. Results:The oral bacterial diversity of patients after radiotherapy significantly differed from that of patients before radiotherapy and their accompanying family members before and after radiotherapy in Observed species, Chao1, Simpson index (all P<0.05). There was a significant difference in Shannon index between the severe and mild ROM patients ( P=0.036). LEfSe analysis showed that patients with severe ROM had higher levels of g_ Streptococcus and f_ Streptococcus, and lower levels of f_ Familyxl, g_ Gemini and o_ Bacillus. The Simpson index and PCoA results in the oral bacterial samples significantly differed between the negative control and radiotherapy groups (all P<0.05). Conclusions:Radiotherapy can disrupt the balance of bacterial flora. The dysregulated oral bacterial flora is closely associated with the aggravation of ROM.
