Application of two-photon imaging technology in the repair evaluation of radiation-induced skin injury in rats
10.3760/cma.j.issn.0254-5098.2021.12.008
- VernacularTitle:双光子成像技术在大鼠放射性皮肤损伤修复评估中的应用
- Author:
Gaiying HE
1
;
Wenfeng GOU
;
Yi WANG
;
Wenbin HOU
;
Jinghui TANG
;
Shuhua MA
;
Yanan SUN
;
Weifeng YANG
;
Qiang FANG
;
Yiliang LI
Author Information
1. 中国中医科学院医学实验中心，北京 100700
- Keywords:
Radiation skin injury;
Two-photon excitation fluorescence imaging;
Epidermal cells;
Collagen fibers;
Damage repair
- From:
Chinese Journal of Radiological Medicine and Protection
2021;41(12):926-930
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the skin development and repair process of X-ray radiation damage in rat with non-invasive two-photon excitation fluorescence (TPEF) imaging technology in vivo. Methods:Totally 24 SD rats were randomly divided into four groups including X-ray irradiated group (25, 35 and 45 Gy) and non-irradiation control group. At different times after irradiation, the degree of skin injury was evaluated, and the pathological changes of nicotinamide adenine dinucleotide (phosphate) [NAD(P)H] and collagen fiber fluorescence signals in epidermal cells were detected in vivo by TPEF imaging technology. Results:At 10 d post-irradiation, the skin of irradiation groups showed erythema and desquamation. At 15-20 d post-irradiation, the skin of radiation groups developed progressive exudation, edema and ulcers with increasing radiation dose. On day 25, the skin began to repair in the 25 Gy group, however, the skin of other groups still had exudation and ulcers. On day 10, NAD(P)H fluorescence signal in epidermal cells of irradiation groups decreased and the fluorescence signal of collagen fibers in papillary layer and reticular layer of irradiation groups reduced, which were significantly lower than that of normal control group ( t=24.145, 28.303, 26.989, 6.654, 7.510, 7.997, P<0.05). On day 30, fluorescence signal of NAD(P)H and collagen fibers in epidermal cells and dermis began to repair, the cell from stratum granulosum, stratum spinosum, and stratum basale in the 25 Gy group showed fluorescence signal, the other groups did not show. The fluorescence signal of collagen fibers in the 25 Gy group were gradually increased in papillary layer and reticular layer, however, they were significantly lower than normal control group ( t=115.133, 17.431, P<0.05), the skin of 45 Gy group did not show fluorescence signal of collagen fibers. Conclusions:The damage and repair process of epidermal cells and dermal collagen fiber can be detected noninvasively by TPEF imaging technology after X-ray irradiation in vivo.
