Protective effect of the Chinese medicine composition on the radon exposure induced injuries in mice lung and bronchial epithelial cells
10.3760/cma.j.issn.0254-5098.2021.08.005
- VernacularTitle:中药组合物对氡暴露小鼠肺组织及支气管上皮细胞损伤的防护作用
- Author:
Yanqing LI
1
;
Qisheng JIANG
;
Gengsheng SHI
;
Siqi TIAN
;
Rong ZHANG
;
Fei CHENG
;
Fengmei CUI
;
Yu TU
;
Huiqin CHEN
Author Information
1. 苏州大学放射医学与防护国家重点实验室 放射医学及交叉学科研究院(RAD-X) 江苏省高校放射医学协同创新中心 215123
- Keywords:
Radon exposure;
Chinese medicinal composition;
Bronchial epithelial cells;
Radiation protection
- From:
Chinese Journal of Radiological Medicine and Protection
2021;41(8):587-594
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of a Chinese medicinal composition ( Xiadanqi) on the prevention of radon exposure induced injuries of lung in vitro and in vivo. Methods:Mice were randomly divided into three groups of blank control group, radon-exposed group alone and radon-exposed group intervened with Chinese medicinal composition. The pathological changes of lung tissues in each group after 120 WLM were observed by HE and Masson staining, and the expressions of α-SMA protein and Vimentin protein in lung tissues were detected by immunohistochemistry staining. The levels of oxidative stress in lung tissue of each group were detected with SOD and MDA kits. At the same time, a radon exposed cell model and a radon exposure + Xiadanqi intervention cell model were constructed using an ecological radon chamber. The cell adhesion abilities of different groups were detected by an adhesion kit. The cell migration ability of each group was determined by the transwell migration experiment. The expression of E-cadherin and Vimentin protein was detected by Western blot. Results:Compared with the radon exposure group, the concentration of MDA was decreased ( t=4.43, P<0.05), the activity of SOD was increased ( t=3.22, P<0.05), and α-SMA and Vimentin protein expressions were decreased ( t=3.08, 7.57, P<0.05) in lung tissue of mice intervened with 2 mg/g Xiadanqi. In vitro, compared with radon exposure group, the migration ability was reduced ( t=4.78, 13.01, P<0.05), the cell adhesion property was enhanced ( t=3.41, 12.55, P<0.05), the expression of E-cadherin protein was increased ( t=2.96, 19.57, P<0.05), and the expression of Vimentin protein was obviously reduced ( t=21.00, 33.32, P<0.05) in radon-exposed cells with the treatment of Chinese medicine (150 μg/ml and 200 μg/ml). Conclusions:The Chinese medicinal composition ( Xiadanqi) has a certain radioprotective effect on radon exposure induced injury by reducing oxidative stress, attenuating EMT and fibrosis, and thus it may be applied as a protective agent for radon induced injury.
