Effect of apatinib on radiosensitivity of glioma cells
10.3760/cma.j.issn.0254-5098.2021.04.003
- VernacularTitle:阿帕替尼对脑胶质瘤细胞放射敏感性的影响
- Author:
Tianyuan LI
1
;
Long HAI
;
Pengfei ZHAO
;
Xiaoxu LU
;
Xueming SUN
;
Rong HUANG
;
Hui WU
Author Information
1. 郑州大学附属肿瘤医院放疗科，郑州 450008
- Keywords:
Glioma cells;
Radiosensitivity;
Apatinib;
Apoptosis
- From:
Chinese Journal of Radiological Medicine and Protection
2021;41(4):253-258
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of apatinib on radiosensitivity of glioma cells U87MG and its potential mechanism.Methods:U87MG cells were divided into control group, apatinib group, radiation group and combination group treated with apatinib and radiation. The effect of different concentrations of apatinib (5, 10, 20, 40, 80 μmol/L) on cell proliferation was detected by CCK8 assay. The effect of apatinib on cell migration and invasion was detected by wound-healing assay and transwell assay, respectively. The effect of apatinib on cell radiosensitivity was detected by plate cloning assay, the cell apoptosis rate was detected by flow cytometry, and the protein expressions of Bax and Bcl-2 were detected by Western blot.Results:Apatinib significantly inhibited the proliferation of U87MG cells in a manner depended on the drug treatment time and radiation. Compared with the radiation group, the cell proliferation, migration and invasion in the combination group were inhibited much significantly ( t=9.857, 18.704, 4.197, P<0.05), so that the value of D0, Dq and SF2 of the combination group was lower, resulting in a radiosensitivity enhancement ratio (SER D0 ) of 1.3. Moreover, compared with the radiation group, the apoptosis rate of the combination group was increased, the expression of Bcl-2 protein was decreased, and the expression of Bax protein was increased ( t=16.187, 8.890, 5.222, P< 0.05). Conclusions:Apatinib inhibits cell proliferation, invasion and migration, induces apoptosis and increases radiosensitivity of glioma cells.
