Effects of calcium sulfate on proliferation of osteoblast-like MG-63 cells and osteoprotegerin/receptor activator of NF-κB ligand/receptor activator of NF-κB system
10.3760/cma.j.cn115530-20210508-00221
- VernacularTitle:硫酸钙对成骨样MG-63细胞增殖及骨保护素/核激活因子受体配体/核激活因子受体系统的影响研究
- Author:
Zhengmao LI
1
;
Bin CHEN
;
Zhi CUI
;
Wenfu TAN
;
Min HE
Author Information
1. 南华大学附属第二医院创伤骨科，湖南省衡阳市 421001
- Keywords:
Calcium sulfate;
Cell proliferation;
Osteogenesis;
Osteoblast-like cells MG-63
- From:
Chinese Journal of Orthopaedic Trauma
2021;23(8):707-710
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of calcium sulfate on the proliferation of osteoblast-like MG-63 cells and the osteoprotegerin/receptor activator of NF-κB ligand/receptor activator of NF-κB (OPG/RANKL/RANK) system.Methods:The extract of calcium sulfate was prepared. The osteoblast-like MG-63 cells were cultured for 24 hours in the medium containing calcium sulfate (the calcium sulfate group) and in the normal medium without calcium sulfate (the blank group), respectively. The growth of osteoblast-like MG-63 cells was observed and their proliferation detected by CCK-8. The mRNA and protein expression levels of OPG/RANKL were detected.Results:The growth of osteoblast-like MG-63 cells was fine in both groups. The CCK-8 test showed that the absorbance value at 24 h was 0.997±0.008 for the calcium sulfate group, significantly higher than that for the blank group (0.640±0.003) ( P<0.001). Respectively, the mRNA expression levels of OPG were 2.834±0.176 and 1.005±0.102 and the mRNA expression levels of RANKL 0.355±0.035 and 1.002±0.068 for the calcium sulfate group and the blank control group, showing statistically significant differences ( P<0.001). The results of Western blot showed that compared with the blank control group, the protein expression of OPG in osteoblast-like MG-63 cells was promoted but the protein expression of RANKL inhibited in the calcium sulfate group. Conclusion:Calcium sulfate may have a positive effect on bone formation, because it can promote the proliferation and activity of osteoblast-like MG-63 cells and regulate the OPG/RANKL/RANK system.
