Changes in expression of Tau protein and phosphorylated Tau protein in neurons after spinal cord ischemia-reperfusion injury in rats
10.3760/cma.j.cn115530-20210429-00210
- VernacularTitle:大鼠脊髓缺血再灌注损伤后神经元Tau蛋白及磷酸化-Tau蛋白表达的变化研究
- Author:
Renbin LI
1
;
Shuyujiong KE
;
Peisheng CHEN
;
Dengke ZHU
;
Gang FU
;
Fengfei LIN
Author Information
1. 福建医科大学教学医院，厦门大学附属福州第二医院骨科 350007
- Keywords:
Reperfusion injury;
Spinal cord;
Neurons;
Tau protein;
Phosphorylated Tau protein
- From:
Chinese Journal of Orthopaedic Trauma
2021;23(7):615-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the changes in the expression of Tau protein and phosphorylated Tau (p-Tau) protein in neurons after spinal cord ischemia-reperfusion injury (SCII).Methods:Ninety-six healthy adult SD rats were randomly divided into a sham operation group ( n=48) and a SCII group ( n=48). Based on the reperfusion time of 3 h, 6 h, 12 h, 24 h, 48 h and 72 h, the SCII group was divided into 6 subgroups ( n=8 per subgroup). Immunohistochemical staining was used to observe the apoptosis of spinal cord neurons in the L 4-L 5 segments and the expression of Tau protein and p-Tau protein. Results:In the sham operation group, the neuron cells were intact, mainly concentrated in the gray matter. Tau protein was seen in a small number of neuron cells, and a small amount of filamentous p-Tau protein in the pernucleus and cytoplasm. There was no significant difference between Tau protein and p-Tau protein expression in neurons at each time point ( P>0.05). In the SCII group, scattered Tau protein was seen in the apoptotic cells while there was a strong positive expression of Tau protein in the non-apoptotic cells. The expression of Tau protein in the SCII group gradually increased after injury, reaching a peak at 48h and plateauing at 72 h, and was significantly different between any 2 time points (except for 72 h) ( P<0.05). In the SCII group, the positive expression of p-Tau protein was observed in the cytoplasm of the apoptotic cells in strips and sheets. It increased rapidly within 6 h but did not change significantly after 6 h, showing no significant difference between any 2 time points afterwards ( P>0.05). There was a statistically significant difference in the expression of Tau protein and p-Tau protein between the SCII group and the sham operation group at each time point ( P<0.05). Conclusion:It is hopeful to reduce the severity of spinal cord injury by regulating the expression of Tau protein and p-Tau protein within 6 to 48 hours after SCII.
