Effects of astragaloside IV-mediated endothelial progenitor cells derived exosomes on the biological function of human endothelial cells damaged by high glucose
10.3760/cma.j.cn431274-20200916-01297
- VernacularTitle:黄芪甲苷介导内皮祖细胞外泌体改善高糖受损人内皮细胞生物学功能的研究
- Author:
Furong ZHU
1
;
Jialun YANG
;
Zhongzhi ZHOU
;
Xue BAI
;
Hui XIAO
;
Qingwen XU
;
Fanxin OUYANG
;
Wu XIONG
Author Information
1. 湖南中医药大学第一附属医院烧伤整形外科，长沙 410007
- Keywords:
Astragaloside IV;
Endothelial progenitor cell;
Exosomes;
Biological function;
Endothelial cells
- From:
Journal of Chinese Physician
2021;23(10):1481-1486
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Astragaloside Ⅳ-mediated Endothelial progenitor cells derived exosomes (EPC-Exos) on the biological function of EPC-Exos damaged by high glicose.Methods:EPCs from human umbilical cord blood were isolated and cultured in vitro. the EPC-Exos secreted by EPCs were extracted by ultracentrifugation combined with ultrafiltration, and identified by specific markers CD9, CD63 and CD81, respectively. After the cells were cultured for 24 hours with AS-IV at 100 mg/L and PBS at the same volume, the morphological characteristics of EPC-Exos were observed by transmission electron microscope. Human endothelial cells were isolated, cultured and identified in vitro. The identified endothelial cells were pretreated with 30 mmol/L glucose for 120 h and randomly divided into experimental group and control group, at the same time set the normal group. The cells were cultured for 24 hours, the effects of EPC-Exos on proliferation, adhesion, migration and angiogenesis of endothelial cells damaged by high glucose were observed by using cell counting kit-8 (CCK-8) Cell Proliferation Assay Kit, cell scratch test, adhesion assay and in vitro angiogenesis assay by Matrigel. Results:Compared with the normal group, the proliferation, migration, adhesion and tubulogenesis of human endothelial cells in the control group were significantly lower ( t=24.35, 6.80, 10.65, 9.62, P<0.05). Compared with the control group, the proliferation, adhesion, migration and tubulogenesis of human endothelial cells in the experimental group were significantly enhanced ( t=30.68, 5.99, 5.40, 8.25, P<0.05). Conclusions:EPC-Exos mediated by AS-Ⅳ can significantly improve the biological function of human endothelial cells damaged by high glucose and has the potential to modulate endothelial neovascularization in diabetic rats.
