Effect of PTOV1 on proliferation and migration of esophageal squamous cell carcinoma cell line
10.3760/cma.j.cn431274-20210409-00399
- VernacularTitle:PTOV1对食管鳞癌细胞系增殖和迁移的影响
- Author:
Yanqiu ZHANG
1
;
Zhen WEN
;
Rong WU
;
Bin CHEN
;
Aimin LENG
Author Information
1. 中南大学湘雅医院消化内科，长沙 410008
- Keywords:
Esophageal neoplasms;
Carcinoma, squamous cell;
PTOV1;
Cell proliferation;
Cell migration
- From:
Journal of Chinese Physician
2021;23(9):1336-1341
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of silencing and overexpression of prostate tumor overexpressed 1 (PTOV1) on the proliferation and migration of esophageal squamous cell lines Ec9706 and TE-1, in order to explore the role of PTOV1 in the development of esophageal cancer.Methods:Esophageal squamous cell carcinoma cell lines were cultured in vitro. Lentivirus transfection was used to knock down the expression of PTOV1 in Ec9706 cells, up regulate the expression of PTOV1 in TE-1 cell lines. The expression of PTOV1 in the above cell lines was detected by Western blot. Scratch test, transwell cell migration test, cell counting kit-8 (CCK8) test and colony formation test were used to study the difference of proliferation and migration ability of homologous esophageal squamous cell carcinoma cells with different expression levels of PTOV1. Results:Western blot showed that the expression of PTOV1 in Ec9706 cells transfected with lentivirus was significantly lower than that in the empty vector group, and the expression of PTOV1 in TE-1 cells transfected with lentivirus was significantly higher than that in the empty vector group, which confirmed that the transfection was successful. After silencing the expression of PTOV1 in Ec9706 cell line, the cell migration rate, the number of transmembrane cells, proliferation ability and colony formation rate decreased compared with the empty vector group ( P<0.05). After overexpression of PTOV1 in TE-1 cell line, the cell migration rate, the number of transmembrane cells, proliferation ability and colony formation rate were higher than those in empty vector group ( P<0.05). Conclusions:PTOV1 can promote cell proliferation and migration in esophageal squamous cell cancer (ESCC) cell line.
